| ofs | hex dump | ascii |
|---|---|---|
| 0000 | ff 57 50 43 cc 15 00 00 01 0a 00 01 00 00 00 00 fb ff 05 00 32 00 fb 01 00 00 ff ff 0e 00 00 00 | .WPC................2........... |
| 0020 | 42 00 00 00 06 00 10 00 00 00 50 00 00 00 ff ff 5a 00 00 00 a6 00 00 00 ff ff 64 00 00 00 00 01 | B.........P.....Z.........d..... |
| 0040 | 00 00 4c 65 74 74 65 72 20 47 6f 74 68 69 63 00 00 33 7c 00 6a 00 00 00 01 00 00 00 00 00 00 00 | ..Letter.Gothic..3|.j........... |
| 0060 | 54 69 6d 65 73 20 4e 65 77 20 52 6f 6d 61 6e 20 42 6f 6c 64 00 53 79 6d 62 6f 6c 00 54 69 6d 65 | Times.New.Roman.Bold.Symbol.Time |
| 0080 | 73 20 4e 65 77 20 52 6f 6d 61 6e 20 49 74 61 6c 69 63 00 4c 65 74 74 65 72 20 47 6f 74 68 69 63 | s.New.Roman.Italic.Letter.Gothic |
| 00a0 | 00 ff ff ff ff ff 48 50 20 4c 61 73 65 72 4a 65 74 20 35 53 69 00 00 00 00 00 00 00 00 00 00 00 | ......HP.LaserJet.5Si........... |
| 00c0 | 00 00 00 00 00 00 00 00 00 00 00 48 50 4c 41 53 35 53 49 2e 50 52 53 00 26 02 64 00 20 1c aa 14 | ...........HPLAS5SI.PRS.&.d..... |
| 00e0 | 48 08 00 00 00 00 10 40 8e 00 f9 99 37 12 fb 01 f0 00 14 01 08 01 5c 01 f0 38 20 5a 7c 58 02 40 | H......@....7.........\..8.Z|X.@ |
| 0100 | 54 69 6d 65 73 20 4e 65 77 20 52 6f 6d 61 6e 00 54 69 6d 65 73 20 4e 65 77 20 52 6f 6d 61 6e 20 | Times.New.Roman.Times.New.Roman. |
| 0120 | 42 6f 6c 64 00 53 79 6d 62 6f 6c 00 54 69 6d 65 73 20 4e 65 77 20 52 6f 6d 61 6e 20 49 74 61 6c | Bold.Symbol.Times.New.Roman.Ital |
| 0140 | 69 63 00 4c 65 74 74 65 72 20 47 6f 74 68 69 63 00 4c 65 74 74 65 72 20 47 6f 74 68 69 63 20 42 | ic.Letter.Gothic.Letter.Gothic.B |
| 0160 | 6f 6c 64 00 00 00 00 00 00 00 58 02 b0 04 08 07 60 09 b8 0b 10 0e 68 10 c0 12 18 15 70 17 c8 19 | old.......X.....`.....h.....p... |
| 0180 | 20 1c 78 1e d0 20 28 23 80 25 d8 27 30 2a 88 2c e0 2e 38 31 90 33 e8 35 40 38 98 3a f0 3c 48 3f | ..x...(#.%.'0*.,..81.3.5@8.:.<H? |
| 01a0 | a0 41 ff ff ff ff ff ff ff ff ff ff ff ff ff ff ff ff ff ff ff ff ff ff 00 00 00 00 00 00 00 00 | .A.............................. |
| 01c0 | 00 00 00 00 00 00 00 00 00 00 00 00 b0 04 ff ff d0 00 04 d0 d1 01 23 00 00 26 02 64 00 20 1c aa | ......................#..&.d.... |
| 01e0 | 14 48 08 00 00 00 00 10 40 8e 00 d7 20 51 10 03 00 00 89 45 58 02 40 23 00 01 d1 fb ff 05 00 32 | .H......@....Q.....EX.@#.......2 |
| 0200 | 00 4e 03 00 00 ff ff 10 00 00 00 2d 02 00 00 0c 00 5a 00 00 00 3d 02 00 00 03 00 0a 00 00 00 97 | .N.........-.....Z...=.......... |
| 0220 | 02 00 00 30 00 ad 00 00 00 a1 02 00 00 00 33 7c 00 6a 00 00 00 01 00 00 00 00 00 00 00 48 50 20 | ...0..........3|.j...........HP. |
| 0240 | 4c 61 73 65 72 4a 65 74 20 35 53 69 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 | LaserJet.5Si.................... |
| 0260 | 00 00 48 50 4c 41 53 35 53 49 2e 50 52 53 00 58 02 4e 00 f4 1a 5c 12 1a 09 00 00 00 10 20 50 00 | ..HPLAS5SI.PRS.X.N...\........P. |
| 0280 | 00 00 00 01 00 01 00 f0 00 14 01 08 01 5c 01 f0 38 20 5a 7c 58 02 50 d0 06 06 00 01 00 06 00 06 | .............\..8.Z|X.P......... |
| 02a0 | d0 ab 00 58 02 4e 00 f4 1a 5c 12 1a 09 00 00 00 10 20 50 00 00 00 00 01 00 01 00 58 02 50 28 00 | ...X.N...\........P........X.P(. |
| 02c0 | c8 19 68 10 48 0d 00 00 11 09 00 00 00 5a 00 0b 01 00 8b 14 36 00 54 00 69 00 6d 00 65 00 73 00 | ..h.H........Z......6.T.i.m.e.s. |
| 02e0 | 20 00 4e 00 65 00 77 00 20 00 52 00 6f 00 6d 00 61 00 6e 00 20 00 52 00 65 00 67 00 75 00 6c 00 | ..N.e.w...R.o.m.a.n...R.e.g.u.l. |
| 0300 | 61 00 72 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 | a.r............................. |
| 0320 | 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 | ................................ |
| 0340 | 00 00 00 00 00 00 00 00 00 00 00 00 58 02 fb ff 05 00 32 00 12 0b 00 00 00 02 4f 02 00 00 80 03 | ............X.....2.......O..... |
| 0360 | 00 00 ff ff 43 00 00 00 cf 05 00 00 01 02 4f 02 00 00 12 06 00 00 02 02 b1 02 00 00 61 08 00 00 | ....C.........O.............a... |
| 0380 | 00 01 22 00 82 00 6d 01 82 01 db 01 2b 02 ff ff ff ff ff ff ff ff ff ff ff ff ff ff ff ff ff ff | .."...m.....+................... |
| 03a0 | ff ff 5e 3a 44 50 64 64 a8 9c 44 44 44 64 70 34 44 34 38 64 64 64 64 64 64 64 64 64 64 38 38 70 | ..^:DPdd..DDDdp4D48dddddddddd88p |
| 03c0 | 70 70 58 b8 90 84 84 90 7c 70 90 90 44 4c 90 7c b0 90 90 70 90 84 70 7c 90 90 bc 90 90 7c 44 38 | ppX.....|p..DL.|...p..p|.....|D8 |
| 03e0 | 44 5c 64 44 58 64 58 64 58 44 64 64 38 38 64 38 9c 64 64 64 64 44 4c 38 64 64 90 64 64 58 60 28 | D\dDXdXdXDdd88d8.ddddDL8dd.ddX`( |
| 0400 | 60 6c e9 44 34 6c 5c 00 00 44 44 44 00 00 00 34 00 44 44 44 44 44 44 00 64 44 64 38 00 90 58 90 | `l.D4l\..DDD...4.DDDDDD.dDd8..X. |
| 0420 | 58 90 58 90 58 90 58 b0 82 84 58 7c 58 7c 58 7c 58 7c 58 44 38 44 38 44 38 44 38 90 64 90 64 90 | X.X.X.X...X|X|X|X|XD8D8D8D8.d.d. |
| 0440 | 64 90 64 90 64 90 64 90 64 90 64 90 64 90 64 90 58 90 64 90 62 90 64 90 64 90 64 70 64 90 58 90 | d.d.d.d.d.d.d.d.X.d.b.d.d.dpd.X. |
| 0460 | 58 90 58 84 58 84 58 84 58 84 58 90 7e 7c 58 7c 58 7c 58 7c 58 90 64 90 64 90 64 90 64 90 64 90 | X.X.X.X.X.X.~|X|X|X|X.d.d.d.d.d. |
| 0480 | 64 90 64 00 00 44 38 44 38 44 38 44 38 8c 70 4c 00 90 64 7c 38 7c 50 7c 38 7c 44 7c 38 90 64 90 | d.d..D8D8D8D8.pL..d|8|P|8|D|8.d. |
| 04a0 | 76 90 64 90 64 90 64 90 64 b0 90 84 44 84 44 84 44 70 4c 70 4c 70 4c 70 4c 7c 54 7c 38 00 00 90 | v.d.d.d.d...D.D.DpLpLpLpL|T|8... |
| 04c0 | 64 90 64 90 64 90 64 90 64 90 64 bc 90 90 64 7c 58 7c 58 7c 58 00 00 90 64 7c 38 90 64 84 44 70 | d.d.d.d.d.d...d|X|X|X...d|8.d.Dp |
| 04e0 | 4c 7c 38 90 64 90 64 90 7e 90 64 90 64 13 34 00 00 00 00 00 64 64 00 00 00 43 00 00 00 00 00 00 | L|8.d.d.~.d.d.4.....dd...C...... |
| 0500 | 24 43 57 8c 8c 8c 8c 8c 8c 8c 8c 8c 8c 8c 8c 8c 8c 8c 8c 8c 8c 8c 8c 8c 8c 8c 8c 8c 8c 8c 8c 8c | $CW............................. |
| 0520 | 8c 8c 8c 8c 8c 8c 8c 8c 8c 8c 8c 8c 8c 8c 8c 8c 8c 8c 8c 78 78 78 78 78 78 78 78 78 78 78 78 78 | ...................xxxxxxxxxxxxx |
| 0540 | 78 78 78 78 78 78 78 78 78 78 78 78 78 78 78 78 78 78 78 78 78 78 78 78 78 78 78 4e 48 78 78 48 | xxxxxxxxxxxxxxxxxxxxxxxxxxxNHxxH |
| 0560 | 00 5c 64 44 58 64 64 64 64 c0 64 38 40 98 98 64 3c 40 98 98 64 98 3c 00 44 44 00 58 58 64 c8 44 | .\dDXdddd.d8@..d<@..d.<.DD.XXd.D |
| 0580 | 44 78 c8 64 64 c4 78 98 c8 48 c8 78 78 48 00 76 a4 a4 70 70 c8 00 00 00 00 00 44 58 00 00 00 00 | Dx.dd.x..H.xxH.v..pp......DX.... |
| 05a0 | 00 00 64 00 00 a8 00 c8 00 00 3c 22 84 84 84 84 98 98 b8 c8 c8 64 98 c8 78 74 6c 64 70 c4 78 78 | ..d.......<".........d..xtldp.xx |
| 05c0 | c8 00 00 00 00 00 00 00 00 00 00 00 00 00 64 54 69 6d 65 73 20 4e 65 77 20 52 6f 6d 61 6e 00 54 | ..............dTimes.New.Roman.T |
| 05e0 | 69 6d 65 73 20 4e 65 77 20 52 6f 6d 61 6e 20 42 6f 6c 64 00 53 79 6d 62 6f 6c 00 54 69 6d 65 73 | imes.New.Roman.Bold.Symbol.Times |
| 0600 | 20 4e 65 77 20 52 6f 6d 61 6e 20 49 74 61 6c 69 63 00 00 01 22 00 82 00 6d 01 82 01 db 01 2b 02 | .New.Roman.Italic..."...m.....+. |
| 0620 | ff ff ff ff ff ff ff ff ff ff ff ff ff ff ff ff ff ff ff ff 5e 3a 44 70 64 64 c8 a8 44 44 44 64 | ....................^:Dpdd..DDDd |
| 0640 | 70 34 44 34 38 64 64 64 64 64 64 64 64 64 64 44 44 70 70 70 64 bc 90 84 90 90 84 7c 9c 9c 4c 64 | p4D48ddddddddddDDpppd......|..Ld |
| 0660 | 9c 84 bc 90 9c 7c 9c 90 70 84 90 90 c8 90 90 84 44 38 44 74 64 44 64 70 58 70 58 44 64 70 38 44 | .....|..p.......D8DtdDdpXpXDdp8D |
| 0680 | 70 38 a8 70 64 70 70 58 4c 44 70 64 90 64 64 58 50 2c 50 68 e9 44 34 68 74 00 00 44 44 44 00 00 | p8.pdppXLDpd.ddXP,Ph.D4ht..DDD.. |
| 06a0 | 00 34 00 44 44 44 44 44 44 00 64 44 70 38 00 90 64 90 64 90 64 90 64 90 64 c8 90 90 58 84 58 84 | .4.DDDDDD.dDp8..d.d.d.d.d...X.X. |
| 06c0 | 58 84 58 84 58 4a 38 4a 38 4a 38 4a 38 90 70 9c 64 9c 64 9c 64 9c 64 90 70 90 70 90 70 90 70 90 | X.X.XJ8J8J8J8.p.d.d.d.d.p.p.p.p. |
| 06e0 | 64 90 64 90 70 9a 64 9c 64 90 64 90 64 7a 70 90 64 90 64 90 64 90 58 90 58 90 58 90 58 90 94 84 | d.d.p.d.d.d.dzp.d.d.d.X.X.X.X... |
| 0700 | 58 84 58 84 58 84 58 9c 64 9c 64 9c 64 9c 64 9c 64 9c 64 9c 70 00 00 4c 38 4c 38 4c 38 4c 38 a2 | X.X.X.X.d.d.d.d.d.d.p..L8L8L8L8. |
| 0720 | 70 64 00 9c 70 84 38 84 5a 84 38 84 50 84 38 90 70 90 90 90 70 90 70 9c 64 9c 64 c6 90 90 58 90 | pd..p.8.Z.8.P.8.p...p.p.d.d...X. |
| 0740 | 58 90 58 70 4c 70 4c 70 4c 70 4c 84 66 84 44 00 00 90 70 90 70 90 70 90 70 90 70 90 70 c8 90 90 | X.XpLpLpLpL.f.D...p.p.p.p.p.p... |
| 0760 | 64 84 58 84 58 84 58 00 00 90 70 84 38 90 70 90 58 70 4c 84 44 90 64 90 64 90 92 9c 64 90 70 13 | d.X.X.X...p.8.p.XpL.D.d.d...d.p. |
| 0780 | 34 00 00 00 00 00 64 64 00 00 00 43 00 00 00 00 00 00 36 43 57 8c 8c 8c 8c 8c 8c 8c 8c 8c 8c 8c | 4.....dd...C......6CW........... |
| 07a0 | 8c 8c 8c 8c 8c 8c 8c 8c 8c 8c 8c 8c 8c 8c 8c 8c 8c 8c 8c 8c 8c 8c 8c 8c 8c 8c 8c 8c 8c 8c 8c 8c | ................................ |
| 07c0 | 8c 8c 8c 8c 8c 78 78 78 78 78 78 78 78 78 78 78 78 78 78 78 78 78 78 78 78 78 78 78 78 78 78 78 | .....xxxxxxxxxxxxxxxxxxxxxxxxxxx |
| 07e0 | 78 78 78 78 78 78 78 78 78 78 78 78 78 4e 48 78 78 48 00 6a 64 44 64 64 64 64 64 c0 64 3c 44 98 | xxxxxxxxxxxxxNHxxH.jdDddddd.d<D. |
| 0800 | 98 64 3c 44 94 94 64 98 3c 00 44 44 00 64 64 64 c8 44 44 78 c8 64 64 c8 78 98 c8 48 c8 78 78 48 | .d<D..d.<.DD.ddd.DDx.dd.x..H.xxH |
| 0820 | 00 7e a4 a4 70 70 c8 00 00 00 00 00 44 64 00 00 00 00 00 00 64 00 00 a8 00 c8 00 00 3c 22 84 84 | .~..pp......Dd......d.......<".. |
| 0840 | 84 84 98 98 b8 c8 c8 64 98 c8 78 76 6c 64 70 c4 78 78 c8 00 00 00 00 00 00 00 00 00 00 00 00 00 | .......d..xvldp.xx.............. |
| 0860 | 64 00 01 22 00 ff ff ff ff ff ff 82 00 d2 00 f6 00 ff ff dd 01 ad 02 ff ff ff ff ff ff ff ff ff | d.."............................ |
| 0880 | ff ff ff 5e 39 44 5d 67 64 ab a0 43 44 44 67 71 33 71 33 39 67 67 67 67 67 67 67 67 67 67 39 39 | ...^9D]gd..CDDgq3q39gggggggggg99 |
| 08a0 | 71 71 71 5b b2 90 7e 89 94 7e 76 90 94 43 4e 8d 7e b5 94 90 73 90 85 6b 7e 94 90 bd 90 90 82 43 | qqq[..~..~v..CN.~...s..k~......C |
| 08c0 | 43 44 64 64 43 59 64 59 64 59 43 64 64 38 38 64 38 9c 64 64 64 64 4a 4e 38 64 64 90 64 64 59 63 | CDddCYdYdYCdd88d8.ddddJN8dd.ddYc |
| 08e0 | 29 63 c8 4e 5f 90 90 64 00 64 64 43 59 51 51 64 64 b0 67 64 64 b2 b2 64 46 64 a2 a2 64 b2 46 00 | )c.N_..d.ddCYQQdd.gdd..dFd..d.F. |
| 0900 | 43 43 00 68 68 64 b2 34 34 c8 c8 64 64 b7 78 98 c8 64 c8 64 90 64 00 76 a4 a4 6f 6f ce 00 00 00 | CC.hhd.44..dd.x..d.d.d.v..oo.... |
| 0920 | 00 00 43 68 00 00 00 00 00 00 64 00 00 bd 00 f1 00 00 46 22 9b 9b 9b 9b 90 90 c8 c8 c8 64 90 c8 | ..Ch......d.......F".........d.. |
| 0940 | c8 68 90 90 b2 b2 c8 c8 87 00 00 00 00 00 00 00 00 00 00 00 00 00 64 e5 b2 71 71 71 93 39 22 00 | .h....................d..qqq.9". |
| 0960 | 71 64 44 44 71 71 71 93 9e 90 93 93 93 cb cb 7c 7c d6 90 90 90 90 90 00 43 c8 64 90 52 6f 64 71 | qdDDqqq........||.......C.d.Rodq |
| 0980 | 38 a9 c8 93 66 33 55 90 59 64 73 8d a4 cb c8 c8 cb cb 7c 7c d6 90 90 90 90 90 9e 93 93 93 93 5a | 8...f3U.Yds.......||...........Z |
| 09a0 | a9 90 90 90 90 c8 c8 9e 9e 9e c8 c8 c8 7c 7c c8 c8 87 00 c8 c8 c8 c8 66 c8 90 90 71 c8 c8 b2 c8 | .............||........f...q.... |
| 09c0 | c8 a4 85 78 9c c8 90 c8 00 00 00 c8 71 00 00 00 00 71 93 00 00 00 00 00 00 00 00 00 00 00 00 00 | ...x........q....q.............. |
| 09e0 | 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 | ................................ |
| 0a00 | 00 00 90 90 90 90 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 93 c8 00 | ................................ |
| 0a20 | 00 00 00 00 00 00 00 00 00 93 00 00 00 00 00 00 00 00 c8 c8 00 00 00 00 00 00 00 00 00 c8 ce 94 | ................................ |
| 0a40 | 82 89 71 00 00 7c 55 7e 66 7e 5a 7e 66 94 7c 98 6b 44 44 95 71 8d 71 b7 76 94 6b 85 65 94 71 9e | ..q..|U~f~Z~f.|.kDD.q.q.v.k.e.q. |
| 0a60 | 71 72 71 7a 7c 00 5a 7e 5a 8e 76 9d 6b 94 71 a4 8d 9e 8d 82 5a 7c 44 44 71 76 76 8d 59 82 00 78 | qrqz|.Z~Z.v.k.q.....Z|DDqvv.Y..x |
| 0a80 | 00 80 7c 93 43 43 64 64 64 64 64 00 00 00 00 00 00 00 00 00 00 64 64 00 00 00 00 00 00 00 00 00 | ..|.CCddddd..........dd......... |
| 0aa0 | 82 82 82 82 82 82 82 82 82 82 82 82 82 82 82 82 82 82 82 5a 5a 5a 5a 5a 5a 5a 7c 7c 7c 7c 7c 7c | ...................ZZZZZZZ|||||| |
| 0ac0 | 7c 7c 7c 7c 7c 7c 7c 7c 7c 7c 7c 7c 7c 7c 44 44 44 44 44 44 44 44 44 44 44 44 71 71 71 71 71 71 | ||||||||||||||DDDDDDDDDDDDqqqqqq |
| 0ae0 | 71 76 76 76 76 76 76 76 76 76 76 76 76 8d 8d 8d 8d 8d 8d 8d 8d 8d 8d 8d 8d 8d 8d 8d 8d 8d 8d 8d | qvvvvvvvvvvvv................... |
| 0b00 | 8d 43 00 00 73 00 00 94 7e 94 44 94 8e 9e 02 a9 59 43 fb ff 05 00 32 00 3c 12 00 00 03 02 4f 02 | .C..s...~.D.....YC....2.<.....O. |
| 0b20 | 00 00 44 0b 00 00 04 02 4f 02 00 00 93 0d 00 00 07 00 79 00 00 00 e2 0f 00 00 ff ff 05 00 00 00 | ..D.....O.........y............. |
| 0b40 | a1 00 00 00 00 01 22 00 82 00 6d 01 82 01 db 01 2b 02 ff ff ff ff ff ff ff ff ff ff ff ff ff ff | ......"...m.....+............... |
| 0b60 | ff ff ff ff ff ff 5e 23 29 30 3c 3c 65 5e 29 29 29 3c 43 1f 29 1f 22 3c 3c 3c 3c 3c 3c 3c 3c 3c | ......^#)0<<e^)))<C.)."<<<<<<<<< |
| 0b80 | 3c 22 22 43 43 43 35 6e 56 4f 4f 56 4a 43 56 56 29 2e 56 4a 6a 56 56 43 56 4f 43 4a 56 56 71 56 | <""CCC5nVOOVJCVV).VJjVVCVOCJVVqV |
| 0ba0 | 56 4a 29 22 29 37 3c 29 35 3c 35 3c 35 29 3c 3c 22 22 3c 22 5e 3c 3c 3c 3c 29 2e 22 3c 3c 56 3c | VJ)")7<)5<5<5)<<""<"^<<<<)."<<V< |
| 0bc0 | 3c 35 3a 18 3a 41 e9 29 1f 41 37 00 00 29 29 29 00 00 00 1f 00 29 29 29 29 29 29 00 3c 29 3c 22 | <5:.:A.).A7..))).....)))))).<)<" |
| 0be0 | 00 56 35 56 35 56 35 56 35 56 35 6a 4e 4f 35 4a 35 4a 35 4a 35 4a 35 29 22 29 22 29 22 29 22 56 | .V5V5V5V5V5jNO5J5J5J5J5)")")")"V |
| 0c00 | 3c 56 3c 56 3c 56 3c 56 3c 56 3c 56 3c 56 3c 56 3c 56 3c 56 35 56 3c 56 3b 56 3c 56 3c 56 3c 43 | <V<V<V<V<V<V<V<V<V<V5V<V;V<V<V<C |
| 0c20 | 3c 56 35 56 35 56 35 4f 35 4f 35 4f 35 4f 35 56 4c 4a 35 4a 35 4a 35 4a 35 56 3c 56 3c 56 3c 56 | <V5V5V5O5O5O5O5VLJ5J5J5J5V<V<V<V |
| 0c40 | 3c 56 3c 56 3c 56 3c 00 00 29 22 29 22 29 22 29 22 54 43 2e 00 56 3c 4a 22 4a 30 4a 22 4a 29 4a | <V<V<V<..)")")")"TC..V<J"J0J"J)J |
| 0c60 | 22 56 3c 56 47 56 3c 56 3c 56 3c 56 3c 6a 56 4f 29 4f 29 4f 29 43 2e 43 2e 43 2e 43 2e 4a 32 4a | "V<VGV<V<V<V<jVO)O)O)C.C.C.C.J2J |
| 0c80 | 22 00 00 56 3c 56 3c 56 3c 56 3c 56 3c 56 3c 71 56 56 3c 4a 35 4a 35 4a 35 00 00 56 3c 4a 22 56 | "..V<V<V<V<V<V<qVV<J5J5J5..V<J"V |
| 0ca0 | 3c 4f 29 43 2e 4a 22 56 3c 56 3c 56 4c 56 3c 56 3c 13 1f 00 00 00 00 00 3c 3c 00 00 00 28 00 00 | <O)C.J"V<V<VLV<V<.......<<...(.. |
| 0cc0 | 00 00 00 00 16 28 57 54 54 54 54 54 54 54 54 54 54 54 54 54 54 54 54 54 54 54 54 54 54 54 54 54 | .....(WTTTTTTTTTTTTTTTTTTTTTTTTT |
| 0ce0 | 54 54 54 54 54 54 54 54 54 54 54 54 54 54 54 54 54 54 54 54 54 54 54 78 78 78 78 78 78 78 78 78 | TTTTTTTTTTTTTTTTTTTTTTTxxxxxxxxx |
| 0d00 | 78 78 78 78 78 78 78 78 78 78 78 78 78 78 78 78 78 78 78 78 78 78 78 78 78 78 78 78 78 78 78 4e | xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxN |
| 0d20 | 2b 48 48 2b 00 37 3c 29 35 3c 3c 3c 3c 73 3c 22 26 5b 5b 3c 24 26 5b 5b 3c 5b 24 00 29 29 00 35 | +HH+.7<)5<<<<s<"&[[<$&[[<[$.)).5 |
| 0d40 | 35 3c 78 29 29 48 78 3c 3c 76 48 5b 78 2b 78 48 48 2b 00 47 62 62 43 43 78 00 00 00 00 00 29 35 | 5<x))Hx<<vH[x+xHH+.GbbCCx.....)5 |
| 0d60 | 00 00 00 00 00 00 3c 00 00 65 00 78 00 00 24 22 4f 4f 4f 4f 5b 5b 6e 78 78 3c 5b 78 48 46 41 3c | ......<..e.x..$"OOOO[[nxx<[xHFA< |
| 0d80 | 43 76 48 48 78 00 00 00 00 00 00 00 00 00 00 00 00 00 3c 00 01 22 00 82 00 6d 01 82 01 db 01 2b | CvHHx.............<.."...m.....+ |
| 0da0 | 02 ff ff ff ff ff ff ff ff ff ff ff ff ff ff ff ff ff ff ff ff 5e 3a 44 54 64 64 a8 9c 44 44 44 | .....................^:DTdd..DDD |
| 0dc0 | 64 88 34 44 34 38 64 64 64 64 64 64 64 64 64 64 44 44 88 88 88 64 b8 7c 7c 84 90 7c 7c 90 90 44 | d.4D48ddddddddddDD...d.||..||..D |
| 0de0 | 58 84 70 a8 84 90 7c 90 7c 64 70 90 7c a8 7c 70 70 4c 38 4c 54 64 44 64 64 58 64 58 38 64 64 38 | X.p...|.|dp.|.|ppL8LTdDddXdX8dd8 |
| 0e00 | 38 58 38 90 64 64 64 64 4c 4c 38 64 58 84 58 58 4c 50 38 50 6c e9 44 34 6c 54 00 00 44 44 44 00 | 8X8.ddddLL8dX.XXLP8Pl.D4lT..DDD. |
| 0e20 | 00 00 34 00 44 44 44 44 44 44 00 64 44 64 38 00 7c 64 7c 64 7c 64 7c 64 7c 64 b0 84 84 58 7c 58 | ..4.DDDDDD.dDd8.|d|d|d|d|d...X|X |
| 0e40 | 7c 58 7c 58 7c 58 44 38 44 38 44 38 44 38 84 64 90 64 90 64 90 64 90 64 90 64 90 64 90 64 90 64 | |X|X|XD8D8D8D8.d.d.d.d.d.d.d.d.d |
| 0e60 | 70 58 7c 64 90 64 90 64 90 64 70 58 90 64 7c 64 7c 64 7c 64 7c 64 84 58 84 58 84 58 84 58 90 78 | pX|d.d.d.dpX.d|d|d|d|d.X.X.X.X.x |
| 0e80 | 7c 58 7c 58 7c 58 7c 58 90 64 90 64 90 64 90 64 90 64 90 64 90 64 00 00 44 38 44 38 44 38 44 38 | |X|X|X|X.d.d.d.d.d.d.d..D8D8D8D8 |
| 0ea0 | 98 64 58 00 84 58 70 38 70 48 70 38 70 40 70 38 84 64 84 74 84 64 84 64 90 64 90 64 bc 84 7c 4c | .dX..Xp8pHp8p@p8.d.t.d.d.d.d..|L |
| 0ec0 | 7c 4c 7c 4c 64 4c 64 4c 64 4c 64 4c 70 48 70 38 00 00 90 64 90 64 90 64 90 64 90 64 90 64 a8 84 | |L|LdLdLdLdLpHp8...d.d.d.d.d.d.. |
| 0ee0 | 70 58 70 4c 70 4c 70 4c 00 00 90 64 70 38 84 64 7c 4c 64 4c 70 38 70 58 70 58 90 78 90 64 90 64 | pXpLpLpL...dp8.d|LdLp8pXpX.x.d.d |
| 0f00 | 13 34 00 00 00 00 00 64 64 00 00 00 43 00 00 00 00 00 00 2c 43 57 8c 8c 8c 8c 8c 8c 8c 8c 8c 8c | .4.....dd...C......,CW.......... |
| 0f20 | 8c 8c 8c 8c 8c 8c 8c 8c 8c 8c 8c 8c 8c 8c 8c 8c 8c 8c 8c 8c 8c 8c 8c 8c 8c 8c 8c 8c 8c 8c 8c 8c | ................................ |
| 0f40 | 8c 8c 8c 8c 8c 8c 64 64 64 64 64 64 64 64 64 64 64 64 64 64 64 64 64 64 64 64 64 64 64 64 64 64 | ......dddddddddddddddddddddddddd |
| 0f60 | 64 64 64 64 64 64 64 64 64 64 64 64 64 64 4e 48 78 78 48 00 68 64 4c 64 64 64 64 64 c4 64 38 40 | ddddddddddddddNHxxH.hdLddddd.d8@ |
| 0f80 | 98 98 64 3c 40 98 98 64 98 3c 00 44 44 00 70 70 64 b0 44 44 78 c8 64 64 c4 78 98 c8 48 c8 78 78 | ..d<@..d.<.DD.ppd.DDx.dd.x..H.xx |
| 0fa0 | 48 00 6b 98 98 64 64 b0 00 00 00 00 00 44 70 00 00 00 00 00 00 64 00 00 a8 00 c8 00 00 3c 22 84 | H.k..dd......Dp......d.......<". |
| 0fc0 | 84 84 84 98 98 b8 c8 c8 64 98 c8 78 74 6c 64 88 c4 78 78 c8 00 00 00 00 00 00 00 00 00 00 00 00 | ........d..xtld..xx............. |
| 0fe0 | 00 64 54 69 6d 65 73 20 4e 65 77 20 52 6f 6d 61 6e 00 54 69 6d 65 73 20 4e 65 77 20 52 6f 6d 61 | .dTimes.New.Roman.Times.New.Roma |
| 1000 | 6e 20 42 6f 6c 64 00 53 79 6d 62 6f 6c 00 54 69 6d 65 73 20 4e 65 77 20 52 6f 6d 61 6e 20 49 74 | n.Bold.Symbol.Times.New.Roman.It |
| 1020 | 61 6c 69 63 00 4c 65 74 74 65 72 20 47 6f 74 68 69 63 00 4c 65 74 74 65 72 20 47 6f 74 68 69 63 | alic.Letter.Gothic.Letter.Gothic |
| 1040 | 20 42 6f 6c 64 00 4c 65 74 74 65 72 20 47 6f 74 68 69 63 20 49 74 61 6c 69 63 00 00 10 20 70 8e | .Bold.Letter.Gothic.Italic....p. |
| 1060 | 00 96 c2 47 00 3b 00 58 02 90 fe fe fe fe fe fe fe ff fe ff ff ff ff ff ff fe ff ff ff ff ff ff | ...G.;.X........................ |
| 1080 | fe fe fe ff ff ff ff ff ff ff ff ff ff ff 92 05 10 00 91 00 37 00 67 00 43 00 39 00 2c 01 01 00 | ....................7.g.C.9.,... |
| 10a0 | 25 00 01 25 74 58 02 67 00 f4 1a 5c 12 1a 09 00 00 00 10 20 50 8e 00 a2 0f b0 02 41 01 58 02 50 | %..%tX.g...\........P......A.X.P |
| 10c0 | fe fe fe fe fe fe fe ff fe ff ff ff fe ff ff fe ff fe ff ff ff ff ff fe ff ff ff ff ff ff ff ff | ................................ |
| 10e0 | ff ff ff ff a2 03 11 00 57 00 21 00 40 00 28 00 23 00 2c 01 01 00 00 00 01 b9 bb 68 01 40 00 f4 | ........W.!.@.(.#.,........h.@.. |
| 1100 | 1a 5c 12 1a 09 00 00 00 10 20 50 8e 00 1c 36 47 00 3b 00 68 01 50 fe fe fe fe fe fe fe ff fe ff | .\........P...6G.;.h.P.......... |
| 1120 | ff ff fe ff ff fe ff ff ff ff ff ff fe fe fe ff ff ff ff ff ff ff ff ff ff ff 5c 04 ff ff 93 00 | ..........................\..... |
| 1140 | 35 00 68 00 43 00 3a 00 2c 01 01 00 2c 00 01 72 a0 58 02 68 00 f4 1a 2a 12 66 08 39 07 00 10 20 | 5.h.C.:.,...,..r.X.h...*.f.9.... |
| 1160 | 78 8e 00 72 0a 47 00 3b 00 58 02 58 fe fe fe fe fe fe fe ff ff ff ff ff fe ff ff fe ff ff ff ff | x..r.G.;.X.X.................... |
| 1180 | ff ff fe fe fe ff ff ff ff ff ff ff ff ff ff ff 2c 0a ff ff 97 00 31 00 64 00 64 00 64 00 2c 01 | ................,.....1.d.d.d.,. |
| 11a0 | 01 00 43 00 01 f3 c3 26 02 64 00 20 1c aa 14 48 08 00 00 00 00 10 40 8e 00 f9 99 37 12 fb 01 58 | ..C....&.d.....H......@....7...X |
| 11c0 | 02 40 fe fe fe fe fe fe fe ff fe ff ff ff 06 ff ff fe ff ff ff ff ff ff ff ff ff ff ff ff ff ff | .@.............................. |
| 11e0 | ff ff ff ff ff ff 6a 0a ff ff 97 00 31 00 64 00 64 00 64 00 2c 01 01 00 51 00 01 fe 31 26 02 64 | ......j.....1.d.d.d.,...Q...1&.d |
| 1200 | 00 20 1c e6 14 02 08 00 00 00 00 10 60 8e 00 e1 15 37 12 fb 01 58 02 80 fe fe fe fe fe fe fe ff | ............`....7...X.......... |
| 1220 | ff ff ff ff ff ff ff fe ff ff ff ff ff ff ff ff ff ff ff ff ff ff ff ff ff ff ff ff fb ff 05 00 | ................................ |
| 1240 | 32 00 00 00 00 00 0f 00 5c 03 00 00 6e 12 00 00 ff ff e1 01 00 00 5b 10 00 00 ff ff 41 00 00 00 | 2.......\...n.........[.....A... |
| 1260 | 60 00 00 00 08 00 02 00 00 00 ca 15 00 00 a2 03 0e 00 91 00 37 00 6a 00 43 00 3a 00 2c 01 01 00 | `...................7.j.C.:.,... |
| 1280 | 00 00 01 79 6e 58 02 6a 00 f4 1a 5c 12 1a 09 00 00 00 10 20 50 8e 00 1c 36 47 00 3b 00 58 02 50 | ...ynX.j...\........P...6G.;.X.P |
| 12a0 | fe fe fe fe fe 03 03 ff 04 ff ff ff 01 ff ff fe ff ff ff ff ff ff 02 fe fe ff ff ff ff ff ff ff | ................................ |
| 12c0 | ff ff ff ff e0 03 0f 00 91 00 37 00 6e 00 43 00 3a 00 2c 01 01 00 10 00 01 3c c2 58 02 6e 00 f4 | ..........7.n.C.:.,......<.X.n.. |
| 12e0 | 1a 34 12 1a 09 00 00 00 10 20 70 8e 00 96 c2 47 00 3b 00 58 02 90 fe fe fe fe fe fe fe ff fe ff | .4........p....G.;.X............ |
| 1300 | ff ff ff ff ff fe ff ff ff ff ff ff fe fe fe ff ff ff ff ff ff ff ff ff ff ff 92 05 10 00 91 00 | ................................ |
| 1320 | 37 00 67 00 43 00 39 00 2c 01 01 00 25 00 01 25 74 58 02 67 00 f4 1a 5c 12 1a 09 00 00 00 10 20 | 7.g.C.9.,...%..%tX.g...\........ |
| 1340 | 50 8e 00 a2 0f b0 02 41 01 58 02 50 fe fe fe fe fe fe fe ff fe ff ff ff fe ff ff fe ff fe ff ff | P......A.X.P.................... |
| 1360 | ff ff ff fe ff ff ff ff ff ff ff ff ff ff ff ff a2 03 11 00 57 00 21 00 40 00 28 00 23 00 2c 01 | ....................W.!.@.(.#.,. |
| 1380 | 01 00 00 00 01 b9 bb 68 01 40 00 f4 1a 5c 12 1a 09 00 00 00 10 20 50 8e 00 1c 36 47 00 3b 00 68 | .......h.@...\........P...6G.;.h |
| 13a0 | 01 50 fe fe fe fe fe fe fe ff fe ff ff ff fe ff ff fe ff ff ff ff ff ff fe fe fe ff ff ff ff ff | .P.............................. |
| 13c0 | ff ff ff ff ff ff 5c 04 ff ff 93 00 35 00 68 00 43 00 3a 00 2c 01 01 00 2c 00 01 72 a0 58 02 68 | ......\.....5.h.C.:.,...,..r.X.h |
| 13e0 | 00 f4 1a 2a 12 66 08 39 07 00 10 20 78 8e 00 72 0a 47 00 3b 00 58 02 58 fe fe fe fe fe fe fe ff | ...*.f.9....x..r.G.;.X.X........ |
| 1400 | ff ff ff ff fe ff ff fe ff ff ff ff ff ff fe fe fe ff ff ff ff ff ff ff ff ff ff ff 2c 0a ff ff | ............................,... |
| 1420 | 97 00 31 00 64 00 64 00 64 00 2c 01 01 00 43 00 01 f3 c3 26 02 64 00 20 1c aa 14 48 08 00 00 00 | ..1.d.d.d.,...C....&.d.....H.... |
| 1440 | 00 10 40 8e 00 f9 99 37 12 fb 01 58 02 40 fe fe fe fe fe 07 07 ff 08 ff ff ff 06 ff ff fe ff ff | ..@....7...X.@.................. |
| 1460 | ff ff ff ff ff ff ff ff ff ff ff ff ff ff ff ff ff ff 6a 0a ff ff 97 00 31 00 64 00 64 00 64 00 | ..................j.....1.d.d.d. |
| 1480 | 2c 01 01 00 51 00 01 fe 31 26 02 64 00 20 1c e6 14 02 08 00 00 00 00 10 60 8e 00 e1 15 37 12 fb | ,...Q...1&.d............`....7.. |
| 14a0 | 01 58 02 80 fe fe fe fe fe fe fe ff ff ff ff ff ff ff ff fe ff ff ff ff ff ff ff ff ff ff ff ff | .X.............................. |
| 14c0 | ff ff ff ff ff ff ff ff 2c 0a ff ff 5a 00 1e 00 3c 00 3c 00 3c 00 2c 01 01 00 43 00 01 33 38 4a | ........,...Z...<.<.<.,...C..38J |
| 14e0 | 01 3c 00 20 1c aa 14 48 08 00 00 00 00 10 40 8e 00 f9 99 37 12 fb 01 68 01 40 fe fe fe fe fe fe | .<.....H......@....7...h.@...... |
| 1500 | fe ff fe ff ff ff fe ff ff fe ff ff ff ff ff ff ff ff ff ff ff ff ff ff ff ff ff ff ff ff a8 0a | ................................ |
| 1520 | ff ff 97 00 31 00 64 00 64 00 64 00 2c 01 01 00 64 00 01 f6 71 26 02 64 00 20 1c aa 14 48 08 fa | ....1.d.d.d.,...d...q&.d.....H.. |
| 1540 | 05 00 00 10 68 8e 00 ad 57 37 12 fb 01 58 02 48 fe fe fe fe fe fe fe ff ff ff ff ff fe ff ff fe | ....h...W7...X.H................ |
| 1560 | ff ff ff ff ff ff ff ff ff ff ff ff ff ff ff ff ff ff ff ff ff ff ff ff 64 2e 20 20 45 61 63 68 | ........................d...Each |
| 1580 | 0d 73 61 6d 70 6c 65 20 6f 66 20 76 69 72 61 6c 20 64 69 6c 75 74 69 6f 6e 20 69 73 20 63 6f 63 | .sample.of.viral.dilution.is.coc |
| 15a0 | 75 6c 74 75 72 65 64 20 77 69 74 68 20 50 48 41 a9 73 74 69 6d 75 6c 61 74 65 64 20 6e 6f 72 6d | ultured.with.PHA.stimulated.norm |
| 15c0 | 61 6c 20 64 6f 6e 6f 72 20 50 00 00 d4 01 0c 00 00 06 97 00 31 00 00 00 0c 00 01 d4 8a d1 01 23 | al.donor.P..........1..........# |
| 15e0 | 00 00 26 02 64 00 20 1c aa 14 48 08 00 00 00 00 10 40 8e 00 f9 99 37 12 fb 01 05 f3 c3 58 02 40 | ..&.d.....H......@....7......X.@ |
| 1600 | 23 00 01 d1 c3 0c c3 4e 45 55 54 52 41 4c 49 5a 49 4e 47 20 41 4e 54 49 42 4f 44 59 20 45 56 41 | #......NEUTRALIZING.ANTIBODY.EVA |
| 1620 | 4c 55 41 54 49 4f 4e 20 49 4e 46 45 43 54 49 56 49 54 59 20 52 45 44 55 43 54 49 4f 4e 20 41 53 | LUATION.INFECTIVITY.REDUCTION.AS |
| 1640 | 53 41 59 0a d4 01 0c 00 00 06 97 00 31 00 c8 00 0c 00 01 d4 39 2f 32 33 2f 39 36 c4 0c c4 0a 0a | SAY.........1.......9/23/96..... |
| 1660 | d4 01 0c 00 00 06 97 00 31 00 58 02 0c 00 01 d4 c3 0c c3 31 2e c2 00 58 02 08 07 08 07 10 00 c2 | ........1.X........1...X........ |
| 1680 | 42 52 49 45 46 20 44 45 53 43 52 49 50 54 49 4f 4e c4 0c c4 c6 28 23 08 07 c6 0a 0a c2 00 58 02 | BRIEF.DESCRIPTION....(#.......X. |
| 16a0 | 08 07 08 07 10 00 c2 54 68 65 20 48 49 56 a9 31 20 6e 65 75 74 72 61 6c 69 7a 69 6e 67 20 61 6e | .......The.HIV.1.neutralizing.an |
| 16c0 | 74 69 62 6f 64 79 20 61 73 73 65 73 73 6d 65 6e 74 20 62 79 20 74 68 65 20 69 6e 66 65 63 74 69 | tibody.assessment.by.the.infecti |
| 16e0 | 76 69 74 79 20 72 65 64 75 63 74 69 6f 6e 0d 61 73 73 61 79 20 65 73 74 69 6d 61 74 65 73 20 74 | vity.reduction.assay.estimates.t |
| 1700 | 68 65 20 72 65 64 75 63 74 69 6f 6e 20 6f 66 20 74 68 65 20 6e 75 6d 62 65 72 20 6f 66 20 69 6e | he.reduction.of.the.number.of.in |
| 1720 | 66 65 63 74 69 6f 75 73 20 75 6e 69 74 73 20 70 65 72 0d 6d 69 6c 6c 69 6c 69 74 65 72 20 28 49 | fectious.units.per.milliliter.(I |
| 1740 | 55 50 4d 4c 29 20 69 6e 20 31 20 6d 4c 20 6f 66 20 76 69 72 61 6c 20 73 74 6f 63 6b 20 64 69 6c | UPML).in.1.mL.of.viral.stock.dil |
| 1760 | 75 74 69 6f 6e 73 20 77 68 65 6e 20 74 72 65 61 74 65 64 20 77 69 74 68 0d 48 49 56 49 47 20 6f | utions.when.treated.with.HIVIG.o |
| 1780 | 72 20 70 61 74 69 65 6e 74 20 73 65 72 75 6d 2e 20 20 54 68 69 73 20 61 73 73 61 79 20 69 73 20 | r.patient.serum...This.assay.is. |
| 17a0 | 70 65 72 66 6f 72 6d 65 64 20 69 6e 20 61 20 32 34 a9 77 65 6c 6c 20 70 6c 61 74 65 20 61 6e 64 | performed.in.a.24.well.plate.and |
| 17c0 | 0d 69 73 20 62 61 73 65 64 20 6f 6e 20 74 68 65 20 41 43 54 47 20 63 6f 6e 73 65 6e 73 75 73 20 | .is.based.on.the.ACTG.consensus. |
| 17e0 | 70 6c 61 73 6d 61 20 71 75 61 6e 74 69 74 61 74 69 76 65 20 63 75 6c 74 75 72 65 20 61 73 73 61 | plasma.quantitative.culture.assa |
| 1800 | 79 2c 20 75 73 69 6e 67 0d 73 69 78 20 35 a9 66 6f 6c 64 20 64 69 6c 75 74 69 6f 6e 73 20 6f 66 | y,.using.six.5.fold.dilutions.of |
| 1820 | 20 76 69 72 61 6c 20 69 73 6f 6c 61 74 65 20 6e 65 75 74 72 61 6c 69 7a 65 64 20 62 79 20 48 49 | .viral.isolate.neutralized.by.HI |
| 1840 | 56 49 47 2c 20 73 65 72 75 6d 20 6f 72 0d 70 6c 61 73 6d 61 2e 20 20 41 20 31 3a 31 32 35 20 66 | VIG,.serum.or.plasma...A.1:125.f |
| 1860 | 69 6e 61 6c 20 63 6f 6e 63 65 6e 74 72 61 74 69 6f 6e 20 6f 66 20 48 49 56 49 47 20 69 73 20 61 | inal.concentration.of.HIVIG.is.a |
| 1880 | 64 64 65 64 20 74 6f 20 65 61 63 68 20 6f 66 20 74 68 65 0d 64 69 6c 75 74 69 6f 6e 73 2e 20 20 | dded.to.each.of.the.dilutions... |
| 18a0 | 57 68 65 6e 20 61 75 74 6f 6c 6f 67 6f 75 73 20 73 65 72 75 6d 20 69 73 20 75 73 65 64 2c 20 31 | When.autologous.serum.is.used,.1 |
| 18c0 | 3a 32 30 20 6f 72 20 31 3a 34 30 20 64 69 6c 75 74 69 6f 6e 20 6f 66 0d 73 65 72 75 6d 20 69 73 | :20.or.1:40.dilution.of.serum.is |
| 18e0 | 20 70 72 65 66 65 72 72 65 64 2e 20 20 45 61 63 68 20 73 61 6d 70 6c 65 20 6f 66 20 76 69 72 61 | .preferred...Each.sample.of.vira |
| 1900 | 6c 20 64 69 6c 75 74 69 6f 6e 20 69 73 20 63 6f 63 75 6c 74 75 72 65 64 20 77 69 74 68 0d 50 48 | l.dilution.is.cocultured.with.PH |
| 1920 | 41 a9 73 74 69 6d 75 6c 61 74 65 64 20 6e 6f 72 6d 61 6c 20 64 6f 6e 6f 72 20 50 42 4d 43 20 66 | A.stimulated.normal.donor.PBMC.f |
| 1940 | 6f 72 20 31 34 20 64 61 79 73 2e 20 20 54 68 65 20 73 75 70 65 72 6e 61 74 61 6e 74 20 66 6f 72 | or.14.days...The.supernatant.for |
| 1960 | 20 65 61 63 68 0d 69 6e 64 69 76 69 64 75 61 6c 20 77 65 6c 6c 20 69 73 20 61 73 73 61 79 65 64 | .each.individual.well.is.assayed |
| 1980 | 20 66 6f 72 20 48 49 56 a9 31 20 70 32 34 20 61 6e 74 69 67 65 6e 20 61 73 20 61 20 64 65 74 65 | .for.HIV.1.p24.antigen.as.a.dete |
| 19a0 | 72 6d 69 6e 61 74 69 6f 6e 20 6f 66 0d 76 69 72 61 6c 20 67 72 6f 77 74 68 2e 20 20 54 68 65 20 | rmination.of.viral.growth...The. |
| 19c0 | 61 6e 74 69 67 65 6e 20 72 65 73 75 6c 74 73 20 66 72 6f 6d 20 65 61 63 68 20 77 65 6c 6c 20 63 | antigen.results.from.each.well.c |
| 19e0 | 61 6e 20 62 65 20 65 76 61 6c 75 61 74 65 64 20 62 79 20 61 0d 73 6f 66 74 77 61 72 65 20 70 72 | an.be.evaluated.by.a.software.pr |
| 1a00 | 6f 67 72 61 6d 20 74 6f 20 64 65 74 65 72 6d 69 6e 65 20 74 68 65 20 49 55 50 4d 4c 20 69 6e 20 | ogram.to.determine.the.IUPML.in. |
| 1a20 | 74 68 65 20 70 72 65 73 65 6e 63 65 20 6f 66 20 48 49 56 49 47 2c 0d 70 61 74 69 65 6e 74 20 73 | the.presence.of.HIVIG,.patient.s |
| 1a40 | 65 72 75 6d 20 6f 72 20 6e 6f 72 6d 61 6c 20 68 75 6d 61 6e 20 73 65 72 75 6d 20 28 63 6f 6e 74 | erum.or.normal.human.serum.(cont |
| 1a60 | 72 6f 6c 29 20 61 63 63 6f 72 64 69 6e 67 20 74 6f 20 74 68 65 20 41 43 54 47 0d 56 69 72 6f 6c | rol).according.to.the.ACTG.Virol |
| 1a80 | 6f 67 79 20 4d 61 6e 75 61 6c 2e 20 20 54 68 65 20 64 69 66 66 65 72 65 6e 63 65 20 62 65 74 77 | ogy.Manual...The.difference.betw |
| 1aa0 | 65 65 6e 20 74 68 65 20 49 55 50 4d 4c 20 77 6f 75 6c 64 20 62 65 20 75 73 65 64 20 74 6f 0d 61 | een.the.IUPML.would.be.used.to.a |
| 1ac0 | 73 73 65 73 73 20 6e 65 75 74 72 61 6c 69 7a 69 6e 67 20 63 61 70 61 62 69 6c 69 74 69 65 73 20 | ssess.neutralizing.capabilities. |
| 1ae0 | 6f 66 20 74 68 65 20 74 65 73 74 20 73 70 65 63 69 6d 65 6e 2e 20 20 42 61 73 65 64 20 6f 6e 0d | of.the.test.specimen...Based.on. |
| 1b00 | 70 72 65 6c 69 6d 69 6e 61 72 79 20 73 74 75 64 69 65 73 2c 20 61 73 73 61 79 20 76 61 72 69 61 | preliminary.studies,.assay.varia |
| 1b20 | 74 69 6f 6e 20 69 73 20 65 78 70 65 63 74 65 64 20 74 6f 20 62 65 20 30 2e 37 20 6c 6f 67 2e c6 | tion.is.expected.to.be.0.7.log.. |
| 1b40 | 28 23 08 07 c6 0a 0a d4 01 0c 00 00 06 97 00 31 00 f8 11 0c 00 01 d4 c3 0c c3 32 2e c2 00 58 02 | (#.............1..........2...X. |
| 1b60 | 08 07 08 07 10 00 c2 53 50 45 43 49 4d 45 4e 20 52 45 51 55 49 52 45 4d 45 4e 54 53 c4 0c c4 c6 | .......SPECIMEN.REQUIREMENTS.... |
| 1b80 | 28 23 08 07 c6 0a 0a d4 01 0c 00 00 06 97 00 31 00 88 13 0c 00 01 d4 c2 00 58 02 08 07 08 07 10 | (#.............1.........X...... |
| 1ba0 | 00 c2 32 2e 31 c2 00 58 02 60 09 60 09 16 00 c2 56 69 72 61 6c 20 73 74 6f 63 6b 20 6f 66 20 61 | ..2.1..X.`.`....Viral.stock.of.a |
| 1bc0 | 75 74 6f 6c 6f 67 6f 75 73 20 6f 72 20 68 65 74 65 72 6f 6c 6f 67 6f 75 73 20 48 49 56 a9 31 20 | utologous.or.heterologous.HIV.1. |
| 1be0 | 74 6f 20 62 65 20 61 73 73 61 79 65 64 2e 20 20 41 0d 70 72 65 74 69 74 65 72 65 64 20 76 69 72 | to.be.assayed...A.pretitered.vir |
| 1c00 | 61 6c 20 73 74 6f 63 6b 20 69 73 20 6e 6f 74 20 6e 65 63 65 73 73 61 72 79 2e 20 20 41 20 76 69 | al.stock.is.not.necessary...A.vi |
| 1c20 | 72 75 73 20 74 69 74 72 61 74 69 6f 6e 20 69 6e 20 74 68 65 0d 70 72 65 73 65 6e 63 65 20 6f 66 | rus.titration.in.the.presence.of |
| 1c40 | 20 6e 6f 72 6d 61 6c 20 68 75 6d 61 6e 20 73 65 72 75 6d 20 69 73 20 69 6e 63 6c 75 64 65 64 20 | .normal.human.serum.is.included. |
| 1c60 | 66 6f 72 20 65 61 63 68 20 76 69 72 75 73 20 61 73 20 70 61 72 74 0d 6f 66 20 74 68 65 20 61 73 | for.each.virus.as.part.of.the.as |
| 1c80 | 73 79 2e 20 20 28 50 6c 65 61 73 65 20 73 65 65 20 c0 20 04 c0 63 6f 6e 74 72 6f 6c 20 73 65 74 | sy...(Please.see.....control.set |
| 1ca0 | c0 1f 04 c0 20 69 6e 20 41 73 73 61 79 20 53 65 74 75 70 20 62 65 6c 6f 77 2e 29 c6 28 23 60 09 | .....in.Assay.Setup.below.).(#`. |
| 1cc0 | c6 0a 0a d4 01 0c 00 00 06 97 00 31 00 70 17 0c 00 01 d4 c2 00 58 02 08 07 08 07 10 00 c2 32 2e | ...........1.p.......X........2. |
| 1ce0 | 32 c2 00 58 02 60 09 60 09 16 00 c2 50 61 74 69 65 6e 74 20 73 65 72 75 6d 2f 70 6c 61 73 6d 61 | 2..X.`.`....Patient.serum/plasma |
| 1d00 | 2e 20 20 41 43 44 20 6f 72 20 43 50 44 a9 41 31 20 74 75 62 65 73 20 61 72 65 20 72 65 63 6f 6d | ...ACD.or.CPD.A1.tubes.are.recom |
| 1d20 | 6d 65 6e 64 65 64 20 66 6f 72 0d 70 6c 61 73 6d 61 20 63 6f 6c 6c 65 63 74 69 6f 6e 2e c6 28 23 | mended.for.plasma.collection..(# |
| 1d40 | 60 09 c6 0a 0a d4 01 0c 00 00 06 97 00 31 00 c8 19 0c 00 01 d4 c3 0c c3 33 2e c2 00 58 02 08 07 | `............1..........3...X... |
| 1d60 | 08 07 10 00 c2 4d 65 74 68 6f 64 6f 6c 6f 67 79 c4 0c c4 c6 28 23 08 07 c6 0a 0a d4 01 0c 00 00 | .....Methodology....(#.......... |
| 1d80 | 06 97 00 31 00 58 1b 0c 00 01 d4 c2 00 58 02 08 07 08 07 10 00 c2 33 2e 31 c2 00 58 02 60 09 60 | ...1.X.......X........3.1..X.`.` |
| 1da0 | 09 16 00 c2 c3 0e c3 43 75 6c 74 75 72 65 20 4d 65 64 69 75 6d c4 0e c4 3a c6 28 23 60 09 c6 0a | .......Culture.Medium...:.(#`... |
| 1dc0 | 0a d4 01 0c 00 00 06 97 00 31 00 e8 1c 0c 00 01 d4 c2 00 58 02 08 07 08 07 10 00 c2 c2 00 58 02 | .........1.........X..........X. |
| 1de0 | 60 09 60 09 16 00 c2 c0 00 04 c0 c2 00 58 02 b8 0b b8 0b 1c 00 c2 52 50 4d 49 a9 31 36 34 30 20 | `.`..........X........RPMI.1640. |
| 1e00 | 77 69 74 68 20 67 6c 75 74 61 6d 69 6e 65 c6 28 23 b8 0b c6 0a d4 01 0c 00 00 06 97 00 31 00 b0 | with.glutamine.(#............1.. |
| 1e20 | 1d 0c 00 01 d4 c2 00 58 02 08 07 08 07 10 00 c2 c2 00 58 02 60 09 60 09 16 00 c2 c0 00 04 c0 c2 | .......X..........X.`.`......... |
| 1e40 | 00 58 02 b8 0b b8 0b 1c 00 c2 32 30 25 20 66 65 74 61 6c 20 62 6f 76 69 6e 65 20 73 65 72 75 6d | .X........20%.fetal.bovine.serum |
| 1e60 | 20 28 68 65 61 74 a9 69 6e 61 63 74 69 76 61 74 65 64 29 c6 28 23 b8 0b c6 0a d4 01 0c 00 00 06 | .(heat.inactivated).(#.......... |
| 1e80 | 97 00 31 00 78 1e 0c 00 01 d4 c2 00 58 02 08 07 08 07 10 00 c2 c2 00 58 02 60 09 60 09 16 00 c2 | ..1.x.......X..........X.`.`.... |
| 1ea0 | c0 00 04 c0 c2 00 58 02 b8 0b b8 0b 1c 00 c2 35 25 20 6e 61 74 75 72 61 6c 2c 20 64 65 6c 65 63 | ......X........5%.natural,.delec |
| 1ec0 | 74 69 6e 61 74 65 64 20 49 4c a9 32 20 28 42 6f 65 68 72 69 6e 67 65 72 20 4d 61 6e 6e 68 65 69 | tinated.IL.2.(Boehringer.Mannhei |
| 1ee0 | 6d 2c 20 43 65 6c 6c 75 6c 61 72 0d 50 72 6f 64 75 63 74 73 20 49 6e 63 2e 2c 20 6f 72 20 50 68 | m,.Cellular.Products.Inc.,.or.Ph |
| 1f00 | 61 72 6d 61 63 69 61 29 c6 28 23 b8 0b c6 0a d4 01 0c 00 00 06 97 00 31 00 08 20 0c 00 01 d4 c2 | armacia).(#............1........ |
| 1f20 | 00 58 02 08 07 08 07 10 00 c2 c2 00 58 02 60 09 60 09 16 00 c2 c0 00 04 c0 c2 00 58 02 b8 0b b8 | .X..........X.`.`..........X.... |
| 1f40 | 0b 1c 00 c2 30 2e 30 30 31 25 20 44 45 41 45 a9 44 65 78 74 72 61 6e 20 6f 72 20 32 20 c0 25 06 | ....0.001%.DEAE.Dextran.or.2..%. |
| 1f60 | c0 67 2f 6d 4c 20 70 6f 6c 79 62 72 65 6e 65 20 28 6f 70 74 69 6f 6e 61 6c 29 c6 28 23 b8 0b c6 | .g/mL.polybrene.(optional).(#... |
| 1f80 | 0a d4 01 0c 00 00 06 97 00 31 00 d0 20 0c 00 01 d4 c2 00 58 02 08 07 08 07 10 00 c2 c2 00 58 02 | .........1.........X..........X. |
| 1fa0 | 60 09 60 09 16 00 c2 c0 00 04 c0 c2 00 58 02 b8 0b b8 0b 1c 00 c2 70 65 6e 69 63 69 6c 6c 69 6e | `.`..........X........penicillin |
| 1fc0 | 20 28 31 30 30 20 75 6e 69 74 73 2f 6d 4c 29 2f 73 74 72 65 70 74 6f 6d 79 63 69 6e 20 28 31 30 | .(100.units/mL)/streptomycin.(10 |
| 1fe0 | 30 20 c0 25 06 c0 67 2f 6d 4c 29 20 6f 72 0d 67 65 6e 74 61 6d 69 63 69 6e 20 28 35 30 20 c0 25 | 0..%..g/mL).or.gentamicin.(50..% |
| 2000 | 06 c0 67 2f 6d 4c 29 2e c6 28 23 b8 0b c6 0a 0a d4 01 0c 00 00 06 97 00 31 00 28 23 0c 00 01 d4 | ..g/mL)..(#.............1.(#.... |
| 2020 | c2 00 58 02 08 07 08 07 10 00 c2 33 2e 32 c2 00 58 02 60 09 60 09 16 00 c2 c3 0e c3 41 73 73 61 | ..X........3.2..X.`.`.......Assa |
| 2040 | 79 20 53 65 74 75 70 c4 0e c4 3a c6 28 23 60 09 c6 0a 0a d4 01 0c 00 00 06 97 00 31 00 b8 24 0c | y.Setup...:.(#`............1..$. |
| 2060 | 00 01 d4 c2 00 58 02 08 07 08 07 10 00 c2 c2 00 58 02 60 09 60 09 16 00 c2 33 2e 32 31 c2 00 58 | .....X..........X.`.`....3.21..X |
| 2080 | 02 b8 0b b8 0b 1c 00 c2 50 72 65 70 61 72 65 20 32 20 73 65 74 73 20 6f 66 20 66 69 76 65 a9 66 | ........Prepare.2.sets.of.five.f |
| 20a0 | 6f 6c 64 20 64 69 6c 75 74 69 6f 6e 73 20 6f 66 20 75 6e 64 69 6c 75 74 65 64 20 76 69 72 75 73 | old.dilutions.of.undiluted.virus |
| 20c0 | 0d 73 74 6f 63 6b 20 28 65 2e 67 2e 20 4d 4e 2c 20 70 72 69 6d 61 72 79 20 69 73 6f 6c 61 74 65 | .stock.(e.g..MN,.primary.isolate |
| 20e0 | 29 2e 20 20 4f 6e 65 20 73 65 74 20 6f 66 20 76 69 72 75 73 0d 64 69 6c 75 74 69 6f 6e 73 20 69 | )...One.set.of.virus.dilutions.i |
| 2100 | 73 20 74 68 65 20 c0 20 04 c0 74 65 73 74 20 73 65 74 c0 1f 04 c0 20 74 6f 20 77 68 69 63 68 20 | s.the.....test.set.....to.which. |
| 2120 | 74 65 73 74 20 61 6e 74 69 62 6f 64 79 20 77 69 6c 6c 20 62 65 0d 61 64 64 65 64 2e 20 20 54 68 | test.antibody.will.be.added...Th |
| 2140 | 65 20 6f 74 68 65 72 20 73 65 74 20 6f 66 20 76 69 72 75 73 20 64 69 6c 75 74 69 6f 6e 73 20 69 | e.other.set.of.virus.dilutions.i |
| 2160 | 73 20 74 68 65 20 c0 20 04 c0 63 6f 6e 74 72 6f 6c 0d 73 65 74 c0 1f 04 c0 20 74 6f 20 77 68 69 | s.the.....control.set.....to.whi |
| 2180 | 63 68 20 63 6f 6e 74 72 6f 6c 20 73 65 72 75 6d 20 77 69 6c 6c 20 62 65 20 61 64 64 65 64 2e c6 | ch.control.serum.will.be.added.. |
| 21a0 | 28 23 b8 0b c6 0a d4 00 1c 00 00 16 a0 28 01 00 00 00 00 00 01 00 00 00 30 2a 30 2a 30 2a b0 04 | (#...........(..........0*0*0*.. |
| 21c0 | b0 04 1c 00 00 d4 8c c5 00 20 03 c5 c2 00 58 02 08 07 08 07 10 00 c2 c2 00 58 02 60 09 60 09 16 | ..............X..........X.`.`.. |
| 21e0 | 00 c2 c2 00 58 02 b8 0b b8 0b 1c 00 c2 54 65 73 74 20 53 65 74 3a c6 28 23 b8 0b c6 0a 0a d4 01 | ....X........Test.Set:.(#....... |
| 2200 | 0c 00 00 06 97 00 31 00 90 01 0c 00 01 d4 c2 00 58 02 08 07 08 07 10 00 c2 c2 00 58 02 60 09 60 | ......1.........X..........X.`.` |
| 2220 | 09 16 00 c2 c2 00 58 02 b8 0b b8 0b 1c 00 c2 61 2e c2 00 58 02 10 0e 10 0e 21 00 c2 50 6c 61 63 | ......X........a...X.....!..Plac |
| 2240 | 65 20 31 30 30 30 20 c0 25 06 c0 4c 20 6f 66 20 75 6e 64 69 6c 75 74 65 64 20 76 69 72 75 73 20 | e.1000..%..L.of.undiluted.virus. |
| 2260 | 69 6e 74 6f 20 61 20 74 75 62 65 20 6c 61 62 65 6c 65 64 0d 76 69 72 75 73 20 73 74 6f 63 6b 2e | into.a.tube.labeled.virus.stock. |
| 2280 | c5 01 20 03 c5 c6 28 23 10 0e c6 0a 0a d4 01 0c 00 00 06 97 00 31 00 e8 03 0c 00 01 d4 c2 00 58 | ......(#.............1.........X |
| 22a0 | 02 08 07 08 07 10 00 c2 c2 00 58 02 60 09 60 09 16 00 c2 c2 00 58 02 b8 0b b8 0b 1c 00 c2 62 2e | ..........X.`.`......X........b. |
| 22c0 | c2 00 58 02 10 0e 10 0e 21 00 c2 41 64 64 20 38 34 30 20 c0 25 06 c0 4c 20 6f 66 20 63 75 6c 74 | ..X.....!..Add.840..%..L.of.cult |
| 22e0 | 75 72 65 20 6d 65 64 69 75 6d 20 74 6f 20 65 61 63 68 20 6f 66 20 73 69 78 20 31 32 20 78 20 37 | ure.medium.to.each.of.six.12.x.7 |
| 2300 | 35 20 6d 6d 0d 73 74 65 72 69 6c 65 20 74 75 62 65 73 20 6c 61 62 65 6c 65 64 20 41 20 74 6f 20 | 5.mm.sterile.tubes.labeled.A.to. |
| 2320 | 46 20 28 31 34 30 20 c0 25 06 c0 4c 20 69 66 20 75 73 69 6e 67 20 39 36 20 77 65 6c 6c 0d 66 6f | F.(140..%..L.if.using.96.well.fo |
| 2340 | 72 6d 61 74 29 2e c6 28 23 10 0e c6 0a 0a d4 01 0c 00 00 06 97 00 31 00 08 07 0c 00 01 d4 c2 00 | rmat)..(#.............1......... |
| 2360 | 58 02 08 07 08 07 10 00 c2 c2 00 58 02 60 09 60 09 16 00 c2 c2 00 58 02 b8 0b b8 0b 1c 00 c2 63 | X..........X.`.`......X........c |
| 2380 | 2e c2 00 58 02 10 0e 10 0e 21 00 c2 54 6f 20 74 75 62 65 20 41 2c 20 61 64 64 20 32 31 30 20 c0 | ...X.....!..To.tube.A,.add.210.. |
| 23a0 | 25 06 c0 4c 20 28 33 35 20 c0 25 06 c0 4c 20 66 6f 72 20 39 36 20 77 65 6c 6c 20 66 6f 72 6d 61 | %..L.(35..%..L.for.96.well.forma |
| 23c0 | 74 29 20 6f 66 0d 74 68 65 20 76 69 72 61 6c 20 73 74 6f 63 6b 20 61 6e 64 20 6d 69 78 20 74 68 | t).of.the.viral.stock.and.mix.th |
| 23e0 | 6f 72 6f 75 67 68 6c 79 2e 20 20 52 65 6d 6f 76 65 20 32 31 30 20 c0 25 06 c0 4c 20 28 33 35 0d | oroughly...Remove.210..%..L.(35. |
| 2400 | c0 25 06 c0 4c 20 66 6f 72 20 39 36 20 77 65 6c 6c 20 66 6f 72 6d 61 74 29 20 66 72 6f 6d 20 74 | .%..L.for.96.well.format).from.t |
| 2420 | 75 62 65 20 41 20 61 6e 64 20 61 64 64 20 74 6f 20 74 75 62 65 20 42 2e 20 0d 52 65 70 65 61 74 | ube.A.and.add.to.tube.B...Repeat |
| 2440 | 20 74 68 69 73 20 70 72 6f 63 65 73 73 20 66 6f 72 20 61 20 74 6f 74 61 6c 20 6f 66 20 73 69 78 | .this.process.for.a.total.of.six |
| 2460 | 20 64 69 6c 75 74 69 6f 6e 73 2e 20 20 54 68 65 0d 72 65 73 75 6c 74 69 6e 67 20 64 69 6c 75 74 | .dilutions...The.resulting.dilut |
| 2480 | 69 6f 6e 20 73 63 68 65 6d 65 20 69 73 20 31 3a 35 20 28 74 75 62 65 20 41 29 2c 20 31 3a 32 35 | ion.scheme.is.1:5.(tube.A),.1:25 |
| 24a0 | 20 28 74 75 62 65 0d 42 29 2c 20 31 3a 31 32 35 20 28 74 75 62 65 20 43 29 2c 20 31 3a 36 32 35 | .(tube.B),.1:125.(tube.C),.1:625 |
| 24c0 | 20 28 74 75 62 65 20 44 29 2c 20 31 3a 33 31 32 35 20 28 74 75 62 65 20 45 29 2c 0d 31 3a 31 35 | .(tube.D),.1:3125.(tube.E),.1:15 |
| 24e0 | 36 32 35 20 28 74 75 62 65 20 46 29 2e 20 20 44 69 73 63 61 72 64 20 32 31 30 20 c0 25 06 c0 4c | 625.(tube.F)...Discard.210..%..L |
| 2500 | 20 6f 72 20 33 35 20 c0 25 06 c0 4c 20 66 6f 72 20 39 36 20 77 65 6c 6c 0d 66 6f 72 6d 61 74 20 | .or.35..%..L.for.96.well.format. |
| 2520 | 66 72 6f 6d 20 74 68 65 20 6c 61 73 74 20 64 69 6c 75 74 69 6f 6e 20 28 74 75 62 65 20 46 29 2e | from.the.last.dilution.(tube.F). |
| 2540 | c6 28 23 10 0e c6 0a 0a c2 00 58 02 08 07 08 07 10 00 c2 c2 00 58 02 60 09 60 09 16 00 c2 c2 00 | .(#.......X..........X.`.`...... |
| 2560 | 58 02 b8 0b b8 0b 1c 00 c2 43 6f 6e 74 72 6f 6c 20 53 65 74 3a c6 28 23 b8 0b c6 0a 0a d4 01 0c | X........Control.Set:.(#........ |
| 2580 | 00 00 06 97 00 31 00 a0 0f 0c 00 01 d4 c2 00 58 02 08 07 08 07 10 00 c2 c2 00 58 02 60 09 60 09 | .....1.........X..........X.`.`. |
| 25a0 | 16 00 c2 c2 00 58 02 b8 0b b8 0b 1c 00 c2 61 2e c2 00 58 02 10 0e 10 0e 21 00 c2 50 6c 61 63 65 | .....X........a...X.....!..Place |
| 25c0 | 20 31 30 30 30 20 c0 25 06 c0 4c 20 6f 66 20 75 6e 64 69 6c 75 74 65 64 20 76 69 72 75 73 20 69 | .1000..%..L.of.undiluted.virus.i |
| 25e0 | 6e 74 6f 20 61 20 74 75 62 65 20 6c 61 62 65 6c 65 64 0d 76 69 72 75 73 20 73 74 6f 63 6b 2e c6 | nto.a.tube.labeled.virus.stock.. |
| 2600 | 28 23 10 0e c6 0a 0a d4 01 0c 00 00 06 97 00 31 00 f8 11 0c 00 01 d4 c2 00 58 02 08 07 08 07 10 | (#.............1.........X...... |
| 2620 | 00 c2 c2 00 58 02 60 09 60 09 16 00 c2 c2 00 58 02 b8 0b b8 0b 1c 00 c2 62 2e c2 00 58 02 10 0e | ....X.`.`......X........b...X... |
| 2640 | 10 0e 21 00 c2 41 64 64 20 38 34 30 20 c0 25 06 c0 4c 20 6f 66 20 63 75 6c 74 75 72 65 20 6d 65 | ..!..Add.840..%..L.of.culture.me |
| 2660 | 64 69 75 6d 20 74 6f 20 65 61 63 68 20 6f 66 20 65 69 67 68 74 20 31 32 20 78 20 37 35 0d 6d 6d | dium.to.each.of.eight.12.x.75.mm |
| 2680 | 20 73 74 65 72 69 6c 65 20 74 75 62 65 73 20 6c 61 62 65 6c 65 64 20 41 c0 1c 04 c0 20 74 6f 20 | .sterile.tubes.labeled.A.....to. |
| 26a0 | 48 c0 1c 04 c0 20 28 6f 72 20 33 35 20 c0 25 06 c0 4c 20 66 6f 72 20 39 36 0d 77 65 6c 6c 20 66 | H.....(or.35..%..L.for.96.well.f |
| 26c0 | 6f 72 6d 61 74 29 2e c6 28 23 10 0e c6 0a 0a d4 01 0c 00 00 06 97 00 31 00 18 15 0c 00 01 d4 c2 | ormat)..(#.............1........ |
| 26e0 | 00 58 02 08 07 08 07 10 00 c2 c2 00 58 02 60 09 60 09 16 00 c2 c2 00 58 02 b8 0b b8 0b 1c 00 c2 | .X..........X.`.`......X........ |
| 2700 | 63 2e c2 00 58 02 10 0e 10 0e 21 00 c2 54 6f 20 74 75 62 65 20 41 c0 1c 04 c0 2c 20 61 64 64 20 | c...X.....!..To.tube.A....,.add. |
| 2720 | 32 31 30 20 c0 25 06 c0 4c 20 28 6f 72 20 33 35 20 c0 25 06 c0 4c 20 66 6f 72 20 39 36 20 77 65 | 210..%..L.(or.35..%..L.for.96.we |
| 2740 | 6c 6c 20 66 6f 72 6d 61 74 29 0d 6f 66 20 74 68 65 20 76 69 72 61 6c 20 73 74 6f 63 6b 20 61 6e | ll.format).of.the.viral.stock.an |
| 2760 | 64 20 6d 69 78 20 74 68 6f 72 6f 75 67 68 6c 79 2e 20 20 52 65 6d 6f 76 65 20 32 31 30 20 c0 25 | d.mix.thoroughly...Remove.210..% |
| 2780 | 06 c0 4c 0d 66 72 6f 6d 20 74 75 62 65 20 41 c0 1c 04 c0 20 61 6e 64 20 61 64 64 20 74 6f 20 74 | ..L.from.tube.A.....and.add.to.t |
| 27a0 | 75 62 65 20 42 c0 1c 04 c0 2e 20 20 52 65 70 65 61 74 20 74 68 69 73 20 70 72 6f 63 65 73 73 0d | ube.B.......Repeat.this.process. |
| 27c0 | 66 6f 72 20 61 20 74 6f 74 61 6c 20 6f 66 20 65 69 67 68 74 20 64 69 6c 75 74 69 6f 6e 73 2e 20 | for.a.total.of.eight.dilutions.. |
| 27e0 | 20 54 68 65 20 72 65 73 75 6c 74 69 6e 67 0d 64 69 6c 75 74 69 6f 6e 20 73 63 68 65 6d 65 20 69 | .The.resulting.dilution.scheme.i |
| 2800 | 73 20 31 3a 35 20 28 74 75 62 65 20 41 c0 1c 04 c0 29 2c 20 31 3a 32 35 20 28 74 75 62 65 20 42 | s.1:5.(tube.A....),.1:25.(tube.B |
| 2820 | c0 1c 04 c0 29 2c 0d 31 3a 31 32 35 20 28 74 75 62 65 20 43 c0 1c 04 c0 29 2c 20 31 3a 36 32 35 | ....),.1:125.(tube.C....),.1:625 |
| 2840 | 20 28 74 75 62 65 20 44 c0 1c 04 c0 29 2c 20 31 3a 33 31 32 35 20 28 74 75 62 65 20 45 c0 1c 04 | .(tube.D....),.1:3125.(tube.E... |
| 2860 | c0 29 2c 0d 31 3a 31 35 36 32 35 20 28 74 75 62 65 20 46 c0 1c 04 c0 29 2c 20 31 3a 37 38 31 32 | .),.1:15625.(tube.F....),.1:7812 |
| 2880 | 35 20 28 74 75 62 65 20 47 c0 1c 04 c0 29 2c 20 31 3a 33 39 30 36 32 35 20 28 74 75 62 65 0d 48 | 5.(tube.G....),.1:390625.(tube.H |
| 28a0 | c0 1c 04 c0 29 2e 20 20 44 69 73 63 61 72 64 20 32 31 30 20 c0 25 06 c0 4c 20 6f 72 20 33 35 20 | ....)...Discard.210..%..L.or.35. |
| 28c0 | c0 25 06 c0 4c 20 66 6f 72 20 39 36 20 77 65 6c 6c 20 66 6f 72 6d 61 74 20 66 72 6f 6d 0d 74 68 | .%..L.for.96.well.format.from.th |
| 28e0 | 65 20 6c 61 73 74 20 64 69 6c 75 74 69 6f 6e 20 28 74 75 62 65 20 48 c0 1c 04 c0 29 2e c6 28 23 | e.last.dilution.(tube.H....)..(# |
| 2900 | 10 0e c6 0a 0a d4 01 0c 00 00 06 97 00 31 00 e8 1c 0c 00 01 d4 c2 00 58 02 08 07 08 07 10 00 c2 | .............1.........X........ |
| 2920 | c2 00 58 02 60 09 60 09 16 00 c2 33 2e 32 32 c2 00 58 02 b8 0b b8 0b 1c 00 c2 54 6f 20 76 69 72 | ..X.`.`....3.22..X........To.vir |
| 2940 | 75 73 20 64 69 6c 75 74 69 6f 6e 20 74 75 62 65 73 20 41 a9 46 20 69 6e 20 74 68 65 20 c0 20 04 | us.dilution.tubes.A.F.in.the.... |
| 2960 | c0 74 65 73 74 20 73 65 74 c0 1f 04 c0 20 61 64 64 0d 6e 65 75 74 72 61 6c 69 7a 69 6e 67 20 61 | .test.set.....add.neutralizing.a |
| 2980 | 6e 74 69 62 6f 64 79 20 28 65 2e 67 2e 20 48 49 56 49 47 2c 20 6d 6f 6e 6f 63 6c 6f 6e 61 6c 20 | ntibody.(e.g..HIVIG,.monoclonal. |
| 29a0 | 61 6e 74 69 62 6f 64 79 29 20 61 74 20 61 0d 70 72 65 a9 64 65 74 65 72 6d 69 6e 65 64 20 64 69 | antibody).at.a.pre.determined.di |
| 29c0 | 6c 75 74 69 6f 6e 2e 20 20 49 6e 20 74 68 65 20 63 61 73 65 20 6f 66 20 48 49 56 49 47 2c 20 61 | lution...In.the.case.of.HIVIG,.a |
| 29e0 | 64 64 20 36 2e 37 20 c0 25 06 c0 4c 0d 6f 66 20 75 6e 64 69 6c 75 74 65 64 20 73 74 6f 63 6b 20 | dd.6.7..%..L.of.undiluted.stock. |
| 2a00 | 48 49 56 49 47 20 74 6f 20 74 68 65 20 38 34 30 20 c0 25 06 c0 4c 20 61 6c 72 65 61 64 79 20 69 | HIVIG.to.the.840..%..L.already.i |
| 2a20 | 6e 20 74 68 65 20 74 75 62 65 2c 0d 66 6f 72 20 61 20 66 69 6e 61 6c 20 64 69 6c 75 74 69 6f 6e | n.the.tube,.for.a.final.dilution |
| 2a40 | 20 6f 66 20 31 3a 31 32 35 20 28 6f 72 20 31 2e 31 20 c0 25 06 c0 4c 20 74 6f 20 31 34 30 20 c0 | .of.1:125.(or.1.1..%..L.to.140.. |
| 2a60 | 25 06 c0 4c 20 69 66 20 75 73 69 6e 67 0d 39 36 20 77 65 6c 6c 20 66 6f 72 6d 61 74 29 2e 20 20 | %..L.if.using.96.well.format)... |
| 2a80 | 41 64 64 20 74 68 65 20 73 61 6d 65 20 63 6f 6e 63 65 6e 74 72 61 74 69 6f 6e 73 20 6f 66 20 70 | Add.the.same.concentrations.of.p |
| 2aa0 | 6f 6f 6c 65 64 0d 6e 6f 72 6d 61 6c 20 68 75 6d 61 6e 20 73 65 72 61 20 28 4e 48 53 29 20 74 6f | ooled.normal.human.sera.(NHS).to |
| 2ac0 | 20 74 75 62 65 73 20 43 c0 1c 04 c0 a9 48 c0 1c 04 c0 20 69 6e 20 74 68 65 20 c0 20 04 c0 63 6f | .tubes.C.....H.....in.the.....co |
| 2ae0 | 6e 74 72 6f 6c 20 73 65 74 c0 1f 04 c0 2e 20 0d 49 6e 63 75 62 61 74 65 20 62 6f 74 68 20 73 65 | ntrol.set.......Incubate.both.se |
| 2b00 | 74 73 20 6f 66 20 74 75 62 65 73 20 66 6f 72 20 31 20 68 6f 75 72 20 61 74 20 33 37 c0 24 06 c0 | ts.of.tubes.for.1.hour.at.37.$.. |
| 2b20 | 43 2e c6 28 23 b8 0b c6 0a 0a d4 01 0c 00 00 06 97 00 31 00 f0 23 0c 00 01 d4 c2 00 58 02 08 07 | C..(#.............1..#......X... |
| 2b40 | 08 07 10 00 c2 c2 00 58 02 60 09 60 09 16 00 c2 33 2e 32 33 c2 00 58 02 b8 0b b8 0b 1c 00 c2 43 | .......X.`.`....3.23..X........C |
| 2b60 | 65 6e 74 72 69 66 75 67 65 20 34 38 20 68 6f 75 72 73 20 6f 6c 64 20 50 48 41 20 73 74 69 6d 75 | entrifuge.48.hours.old.PHA.stimu |
| 2b80 | 6c 61 74 65 64 20 6e 6f 72 6d 61 6c 20 64 6f 6e 6f 72 20 50 42 4d 43 20 61 74 0d 34 30 30 20 78 | lated.normal.donor.PBMC.at.400.x |
| 2ba0 | 20 67 20 66 6f 72 20 31 30 20 6d 69 6e 75 74 65 73 20 61 74 20 32 30 20 74 6f 20 32 34 c0 24 06 | .g.for.10.minutes.at.20.to.24.$. |
| 2bc0 | c0 43 2c 20 72 65 6d 6f 76 65 20 61 6e 64 20 64 69 73 63 61 72 64 0d 73 75 70 65 72 6e 61 74 61 | .C,.remove.and.discard.supernata |
| 2be0 | 6e 74 2e 20 20 52 65 73 75 73 70 65 6e 64 20 63 65 6c 6c 73 20 69 6e 20 63 75 6c 74 75 72 65 20 | nt...Resuspend.cells.in.culture. |
| 2c00 | 6d 65 64 69 75 6d 20 61 6e 64 0d 65 6e 75 6d 65 72 61 74 65 20 63 65 6c 6c 73 2e 20 20 41 64 6a | medium.and.enumerate.cells...Adj |
| 2c20 | 75 73 74 20 73 61 6d 70 6c 65 20 77 69 74 68 20 63 75 6c 74 75 72 65 20 6d 65 64 69 75 6d 20 74 | ust.sample.with.culture.medium.t |
| 2c40 | 6f 20 61 0d d4 01 0c 00 00 06 97 00 31 00 10 27 0c 00 01 d4 63 6f 6e 63 65 6e 74 72 61 74 69 6f | o.a.........1..'....concentratio |
| 2c60 | 6e 20 6f 66 20 31 2e 32 35 20 78 20 31 30 c3 05 c3 36 c4 05 c4 20 50 42 4d 43 2f 6d 4c 2e c6 28 | n.of.1.25.x.10...6....PBMC/mL..( |
| 2c80 | 23 b8 0b c6 0a d4 00 1c 00 00 16 d8 27 02 00 00 00 00 00 01 00 00 00 30 2a 30 2a 30 2a b0 04 b0 | #...........'..........0*0*0*... |
| 2ca0 | 04 1c 00 00 d4 8c d4 01 0c 00 00 06 97 00 31 00 00 00 0c 00 01 d4 c5 00 20 03 c5 c2 00 58 02 08 | ..............1..............X.. |
| 2cc0 | 07 08 07 10 00 c2 c2 00 58 02 60 09 60 09 16 00 c2 33 2e 32 34 c2 00 58 02 b8 0b b8 0b 1c 00 c2 | ........X.`.`....3.24..X........ |
| 2ce0 | 53 65 74 20 75 70 20 32 20 73 65 74 73 20 6f 66 20 50 42 4d 43 20 73 74 65 72 69 6c 65 20 74 75 | Set.up.2.sets.of.PBMC.sterile.tu |
| 2d00 | 62 65 73 20 28 35 20 6d 4c 29 20 6f 72 20 77 65 6c 6c 73 20 28 32 34 a9 77 65 6c 6c 0d 6f 72 20 | bes.(5.mL).or.wells.(24.well.or. |
| 2d20 | 39 36 20 77 65 6c 6c 20 70 6c 61 74 65 2c 20 69 66 20 79 6f 75 20 63 61 6e 20 63 65 6e 74 72 69 | 96.well.plate,.if.you.can.centri |
| 2d40 | 66 75 67 65 20 70 6c 61 74 65 73 29 2c 0d 63 6f 72 72 65 73 70 6f 6e 64 69 6e 67 20 74 6f 20 74 | fuge.plates),.corresponding.to.t |
| 2d60 | 68 65 20 c0 20 04 c0 74 65 73 74 20 73 65 74 c0 1f 04 c0 20 6f 66 20 76 69 72 75 73 2f 48 49 56 | he.....test.set.....of.virus/HIV |
| 2d80 | 49 47 20 74 75 62 65 73 20 61 6e 64 20 74 68 65 0d c0 20 04 c0 63 6f 6e 74 72 6f 6c 20 74 65 73 | IG.tubes.and.the.....control.tes |
| 2da0 | 74 c0 1f 04 c0 20 6f 66 20 76 69 72 75 73 2f 4e 48 53 20 74 75 62 65 73 2e c5 01 20 03 c5 c6 28 | t.....of.virus/NHS.tubes.......( |
| 2dc0 | 23 b8 0b c6 0a 0a d4 01 0c 00 00 06 97 00 31 00 e8 03 0c 00 01 d4 c2 00 58 02 08 07 08 07 10 00 | #.............1.........X....... |
| 2de0 | c2 c2 00 58 02 60 09 60 09 16 00 c2 c2 00 58 02 b8 0b b8 0b 1c 00 c2 61 2e c2 00 58 02 10 0e 10 | ...X.`.`......X........a...X.... |
| 2e00 | 0e 21 00 c2 41 64 64 20 33 2e 32 20 6d 4c 20 6f 66 20 61 62 6f 76 65 20 50 48 41 a9 73 74 69 6d | .!..Add.3.2.mL.of.above.PHA.stim |
| 2e20 | 75 6c 61 74 65 64 20 50 42 4d 43 20 74 6f 20 65 61 63 68 20 74 75 62 65 0d 6f 72 20 31 2e 36 20 | ulated.PBMC.to.each.tube.or.1.6. |
| 2e40 | 6d 4c 20 6f 66 20 50 42 4d 43 20 74 6f 20 65 61 63 68 20 77 65 6c 6c 20 28 32 34 20 77 65 6c 6c | mL.of.PBMC.to.each.well.(24.well |
| 2e60 | 20 70 6c 61 74 65 29 20 6f 72 20 31 36 30 0d c0 25 06 c0 4c 20 28 39 36 20 77 65 6c 6c 20 70 6c | .plate).or.160..%..L.(96.well.pl |
| 2e80 | 61 74 65 29 2e c6 28 23 10 0e c6 0a 0a d4 01 0c 00 00 06 97 00 31 00 08 07 0c 00 01 d4 c2 00 58 | ate)..(#.............1.........X |
| 2ea0 | 02 08 07 08 07 10 00 c2 c2 00 58 02 60 09 60 09 16 00 c2 c2 00 58 02 b8 0b b8 0b 1c 00 c2 62 2e | ..........X.`.`......X........b. |
| 2ec0 | c2 00 58 02 10 0e 10 0e 21 00 c2 44 69 73 70 65 6e 73 65 20 38 30 30 20 c0 25 06 c0 4c 20 6f 66 | ..X.....!..Dispense.800..%..L.of |
| 2ee0 | 20 61 70 70 72 6f 70 72 69 61 74 65 20 76 69 72 75 73 2f 48 49 56 49 47 20 6f 72 0d 76 69 72 75 | .appropriate.virus/HIVIG.or.viru |
| 2f00 | 73 2f 4e 48 53 20 64 69 6c 75 74 69 6f 6e 20 74 6f 20 63 6f 72 72 65 73 70 6f 6e 64 69 6e 67 20 | s/NHS.dilution.to.corresponding. |
| 2f20 | 50 42 4d 43 20 74 75 62 65 73 2c 20 6f 72 20 34 30 30 0d c0 25 06 c0 4c 20 74 6f 20 65 61 63 68 | PBMC.tubes,.or.400..%..L.to.each |
| 2f40 | 20 6f 66 20 74 77 6f 20 63 6f 72 72 65 73 70 6f 6e 64 69 6e 67 20 77 65 6c 6c 73 20 28 32 34 20 | .of.two.corresponding.wells.(24. |
| 2f60 | 77 65 6c 6c 20 70 6c 61 74 65 29 0d 6f 72 20 34 30 20 c0 25 06 c0 4c 20 74 6f 20 65 61 63 68 20 | well.plate).or.40..%..L.to.each. |
| 2f80 | 77 65 6c 6c 20 28 39 36 20 77 65 6c 6c 20 70 6c 61 74 65 29 2e c6 28 23 10 0e c6 0a 0a d4 01 0c | well.(96.well.plate)..(#........ |
| 2fa0 | 00 00 06 97 00 31 00 f0 0a 0c 00 01 d4 c2 00 58 02 08 07 08 07 10 00 c2 c2 00 58 02 60 09 60 09 | .....1.........X..........X.`.`. |
| 2fc0 | 16 00 c2 33 2e 32 35 c2 00 58 02 b8 0b b8 0b 1c 00 c2 50 6c 61 63 65 20 74 68 65 20 74 75 62 65 | ...3.25..X........Place.the.tube |
| 2fe0 | 73 20 6f 72 20 70 6c 61 74 65 20 69 6e 74 6f 20 61 20 35 25 20 43 4f c3 06 c3 32 c4 06 c4 2c 20 | s.or.plate.into.a.5%.CO...2...,. |
| 3000 | 68 75 6d 69 64 69 66 69 65 64 20 63 68 61 6d 62 65 72 0d 6f 72 20 63 6f 76 65 72 20 74 68 65 20 | humidified.chamber.or.cover.the. |
| 3020 | 70 6c 61 74 65 20 77 69 74 68 20 61 20 35 20 78 20 38 20 69 6e 63 68 20 6c 6f 77 a9 64 65 6e 73 | plate.with.a.5.x.8.inch.low.dens |
| 3040 | 69 74 79 0d 70 6f 6c 79 65 74 68 79 6c 65 6e 65 20 62 61 67 20 28 4e 61 6c 67 65 6e 65 20 36 32 | ity.polyethylene.bag.(Nalgene.62 |
| 3060 | 35 35 a9 30 35 30 38 38 29 2e 20 20 49 6e 63 75 62 61 74 65 20 6f 76 65 72 6e 69 67 68 74 0d 74 | 55.05088)...Incubate.overnight.t |
| 3080 | 68 65 20 73 61 6d 70 6c 65 73 20 61 74 20 33 37 c0 24 06 c0 43 2e 20 20 54 68 69 73 20 69 73 20 | he.samples.at.37.$..C...This.is. |
| 30a0 | 64 61 79 20 30 2e c6 28 23 b8 0b c6 0a 0a d4 01 0c 00 00 06 97 00 31 00 d8 0e 0c 00 01 d4 c2 00 | day.0..(#.............1......... |
| 30c0 | 58 02 08 07 08 07 10 00 c2 c2 00 58 02 60 09 60 09 16 00 c2 33 2e 32 36 c2 00 58 02 b8 0b b8 0b | X..........X.`.`....3.26..X..... |
| 30e0 | 1c 00 c2 44 61 79 20 31 2e 20 20 41 66 74 65 72 20 6f 76 65 72 6e 69 67 68 74 20 69 6e 63 75 62 | ...Day.1...After.overnight.incub |
| 3100 | 61 74 69 6f 6e 2c 20 77 61 73 68 20 63 65 6c 6c 73 20 32 20 74 69 6d 65 73 20 28 34 30 30 0d 78 | ation,.wash.cells.2.times.(400.x |
| 3120 | 20 67 20 66 6f 72 20 31 30 20 6d 69 6e 75 74 65 73 29 20 77 69 74 68 20 63 75 6c 74 75 72 65 20 | .g.for.10.minutes).with.culture. |
| 3140 | 6d 65 64 69 75 6d 20 69 6e 20 74 75 62 65 73 20 6f 72 20 77 65 6c 6c 73 2e 20 0d 49 66 20 75 73 | medium.in.tubes.or.wells...If.us |
| 3160 | 69 6e 67 20 74 68 65 20 77 65 6c 6c 20 6d 65 74 68 6f 64 2c 20 61 66 74 65 72 20 74 68 65 20 6c | ing.the.well.method,.after.the.l |
| 3180 | 61 73 74 20 77 61 73 68 20 72 65 73 75 73 70 65 6e 64 20 69 6e 20 32 0d 6d 4c 20 28 32 34 20 77 | ast.wash.resuspend.in.2.mL.(24.w |
| 31a0 | 65 6c 6c 73 29 20 6f 72 20 32 30 30 20 c0 25 06 c0 4c 20 28 39 36 20 77 65 6c 6c 73 29 20 6f 66 | ells).or.200..%..L.(96.wells).of |
| 31c0 | 20 66 72 65 73 68 20 63 75 6c 74 75 72 65 20 6d 65 64 69 75 6d 0d 61 6e 64 20 72 65 74 75 72 6e | .fresh.culture.medium.and.return |
| 31e0 | 20 74 6f 20 74 68 65 20 63 6f 72 72 65 73 70 6f 6e 64 69 6e 67 20 77 65 6c 6c 2e 20 20 49 66 20 | .to.the.corresponding.well...If. |
| 3200 | 75 73 69 6e 67 20 74 68 65 20 74 75 62 65 0d 6d 65 74 68 6f 64 2c 20 61 66 74 65 72 20 74 68 65 | using.the.tube.method,.after.the |
| 3220 | 20 6c 61 73 74 20 77 61 73 68 20 72 65 73 75 73 70 65 6e 64 20 69 6e 20 34 20 6d 4c 20 6f 66 20 | .last.wash.resuspend.in.4.mL.of. |
| 3240 | 66 72 65 73 68 0d 70 6c 61 73 6d 61 20 63 75 6c 74 75 72 65 20 6d 65 64 69 75 6d 20 61 6e 64 20 | fresh.plasma.culture.medium.and. |
| 3260 | 72 65 74 75 72 6e 20 74 6f 20 74 68 65 20 63 6f 72 72 65 73 70 6f 6e 64 69 6e 67 20 74 75 62 65 | return.to.the.corresponding.tube |
| 3280 | 2e 20 0d 54 72 61 6e 73 66 65 72 20 63 65 6c 6c 73 20 66 72 6f 6d 20 63 6f 72 72 65 73 70 6f 6e | ...Transfer.cells.from.correspon |
| 32a0 | 64 69 6e 67 20 74 75 62 65 73 20 74 6f 20 77 65 6c 6c 73 20 6f 6e 20 61 20 32 34 0d 77 65 6c 6c | ding.tubes.to.wells.on.a.24.well |
| 32c0 | 20 70 6c 61 74 65 20 28 74 75 62 65 20 41 20 74 6f 20 77 65 6c 6c 73 20 41 31 2c 20 41 32 3b 20 | .plate.(tube.A.to.wells.A1,.A2;. |
| 32e0 | 74 75 62 65 20 42 20 74 6f 20 77 65 6c 6c 73 20 42 31 2c 20 42 32 2c 0d 65 74 63 2e 29 2e c6 28 | tube.B.to.wells.B1,.B2,.etc.)..( |
| 3300 | 23 b8 0b c6 0a 0a d4 01 0c 00 00 06 97 00 31 00 70 17 0c 00 01 d4 c2 00 58 02 08 07 08 07 10 00 | #.............1.p.......X....... |
| 3320 | c2 c2 00 58 02 60 09 60 09 16 00 c2 33 2e 32 37 c2 00 58 02 b8 0b b8 0b 1c 00 c2 4f 6e 20 64 61 | ...X.`.`....3.27..X........On.da |
| 3340 | 79 20 37 2c 20 72 65 6d 6f 76 65 20 6f 6e 65 20 68 61 6c 66 20 6f 66 20 74 68 65 20 6d 65 64 69 | y.7,.remove.one.half.of.the.medi |
| 3360 | 75 6d 20 28 31 20 6d 4c 29 20 6f 72 20 31 30 30 20 c0 25 06 c0 4c 20 69 66 0d 39 36 20 77 65 6c | um.(1.mL).or.100..%..L.if.96.wel |
| 3380 | 6c 20 70 6c 61 74 65 73 20 77 65 72 65 20 75 73 65 64 20 61 6e 64 20 73 61 76 65 20 66 6f 72 20 | l.plates.were.used.and.save.for. |
| 33a0 | 66 75 74 75 72 65 20 74 65 73 74 69 6e 67 20 28 73 61 76 65 0d 61 74 20 a9 37 30 c0 24 06 c0 43 | future.testing.(save.at..70.$..C |
| 33c0 | 29 20 61 6e 64 20 72 65 70 6c 61 63 65 20 77 69 74 68 20 31 20 6d 4c 20 6f 66 20 66 72 65 73 68 | ).and.replace.with.1.mL.of.fresh |
| 33e0 | 20 63 75 6c 74 75 72 65 20 6d 65 64 69 75 6d 0d d4 01 0c 00 00 06 97 00 31 00 c8 19 0c 00 01 d4 | .culture.medium.........1....... |
| 3400 | 63 6f 6e 74 61 69 6e 69 6e 67 20 30 2e 35 20 78 20 31 30 c3 05 c3 36 c4 05 c4 2c 20 34 38 a9 68 | containing.0.5.x.10...6...,.48.h |
| 3420 | 6f 75 72 20 50 48 41 a9 73 74 69 6d 75 6c 61 74 65 64 20 6e 6f 72 6d 61 6c 20 64 6f 6e 6f 72 0d | our.PHA.stimulated.normal.donor. |
| 3440 | 50 42 4d 43 20 6f 72 20 31 30 30 20 c0 25 06 c0 4c 20 69 66 20 75 73 69 6e 67 20 39 36 20 77 65 | PBMC.or.100..%..L.if.using.96.we |
| 3460 | 6c 6c 20 70 6c 61 74 65 73 2e 20 20 43 75 6c 74 75 72 65 73 20 61 72 65 0d 74 65 72 6d 69 6e 61 | ll.plates...Cultures.are.termina |
| 3480 | 74 65 64 20 6f 6e 20 64 61 79 20 31 34 2c 20 61 74 20 77 68 69 63 68 20 74 69 6d 65 20 31 2e 30 | ted.on.day.14,.at.which.time.1.0 |
| 34a0 | 20 6d 4c 20 6f 66 20 6d 65 64 69 75 6d 20 69 73 0d 77 69 74 68 64 72 61 77 6e 20 61 6e 64 20 73 | .mL.of.medium.is.withdrawn.and.s |
| 34c0 | 74 6f 72 65 64 20 61 74 20 a9 37 30 c0 24 06 c0 43 20 66 6f 72 20 73 75 62 73 65 71 75 65 6e 74 | tored.at..70.$..C.for.subsequent |
| 34e0 | 20 48 49 56 20 70 32 34 20 61 6e 74 69 67 65 6e 0d 64 65 74 65 72 6d 69 6e 61 74 69 6f 6e 2e c6 | .HIV.p24.antigen.determination.. |
| 3500 | 28 23 b8 0b c6 0a 0a c2 00 58 02 08 07 08 07 10 00 c2 c2 00 58 02 60 09 60 09 16 00 c2 c2 00 58 | (#.......X..........X.`.`......X |
| 3520 | 02 b8 0b b8 0b 1c 00 c2 46 6f 72 20 74 68 65 20 6c 61 62 73 20 75 73 69 6e 67 20 74 68 65 20 39 | ........For.the.labs.using.the.9 |
| 3540 | 36 20 77 65 6c 6c 20 66 6f 72 6d 61 74 2c 20 74 68 65 20 31 30 30 20 c0 25 06 c0 4c 20 68 61 72 | 6.well.format,.the.100..%..L.har |
| 3560 | 76 65 73 74 65 64 0d 65 69 74 68 65 72 20 6f 6e 20 64 61 79 20 37 20 6f 72 20 64 61 79 20 31 34 | vested.either.on.day.7.or.day.14 |
| 3580 | 20 77 69 6c 6c 20 62 65 20 70 75 74 20 69 6e 20 39 36 20 77 65 6c 6c 20 70 6c 61 74 65 73 20 61 | .will.be.put.in.96.well.plates.a |
| 35a0 | 6e 64 0d 6d 69 78 65 64 20 77 69 74 68 20 31 30 30 20 c0 25 06 c0 4c 20 6f 66 20 73 65 72 75 6d | nd.mixed.with.100..%..L.of.serum |
| 35c0 | 20 63 6f 6e 74 61 69 6e 69 6e 67 20 6d 65 64 69 61 20 63 6f 76 65 72 20 74 68 65 20 70 6c 61 74 | .containing.media.cover.the.plat |
| 35e0 | 65 73 0d 77 69 74 68 20 73 65 61 6c 65 72 20 61 6e 64 20 66 72 65 65 7a 65 20 69 6e 20 a9 37 30 | es.with.sealer.and.freeze.in..70 |
| 3600 | c0 24 06 c0 43 2e c6 28 23 b8 0b c6 0a 0a c2 00 58 02 08 07 08 07 10 00 c2 c2 00 58 02 60 09 60 | .$..C..(#.......X..........X.`.` |
| 3620 | 09 16 00 c2 c2 00 58 02 b8 0b b8 0b 1c 00 c2 4e 6f 74 65 3a 20 44 61 79 20 37 20 63 75 6c 74 75 | ......X........Note:.Day.7.cultu |
| 3640 | 72 65 20 73 75 70 65 72 6e 61 74 61 6e 74 73 20 73 68 6f 75 6c 64 20 62 65 20 74 65 73 74 65 64 | re.supernatants.should.be.tested |
| 3660 | 20 69 66 20 64 61 79 20 31 34 0d 73 68 6f 77 73 20 76 69 72 75 73 20 6f 76 65 72 67 72 6f 77 74 | .if.day.14.shows.virus.overgrowt |
| 3680 | 68 20 28 6c 61 63 6b 20 6f 66 20 61 6e 79 20 6e 65 75 74 72 61 6c 69 7a 61 74 69 6f 6e 29 2e c6 | h.(lack.of.any.neutralization).. |
| 36a0 | 28 23 b8 0b c6 0a 0a d4 01 0c 00 00 06 97 00 31 00 b8 24 0c 00 01 d4 c2 00 58 02 08 07 08 07 10 | (#.............1..$......X...... |
| 36c0 | 00 c2 c2 00 58 02 60 09 60 09 16 00 c2 4e 6f 74 65 3a c2 00 58 02 b8 0b b8 0b 1c 00 c2 48 49 56 | ....X.`.`....Note:..X........HIV |
| 36e0 | 49 47 2c 20 4d 4e 20 61 6e 64 20 6e 6f 72 6d 61 6c 20 68 75 6d 61 6e 20 73 65 72 75 6d 20 77 69 | IG,.MN.and.normal.human.serum.wi |
| 3700 | 6c 6c 20 6c 69 6b 65 6c 79 20 62 65 20 63 6f 6e 73 69 64 65 72 65 64 0d 51 75 61 6c 69 74 79 20 | ll.likely.be.considered.Quality. |
| 3720 | 43 6f 6e 74 72 6f 6c 20 4d 61 74 65 72 69 61 6c 73 20 61 6e 64 20 77 69 6c 6c 20 62 65 20 70 72 | Control.Materials.and.will.be.pr |
| 3740 | 6f 76 69 64 65 64 20 63 65 6e 74 72 61 6c 6c 79 20 62 79 0d 74 68 65 20 56 51 41 2e c6 28 23 b8 | ovided.centrally.by.the.VQA..(#. |
| 3760 | 0b c6 0a d4 00 1c 00 00 16 10 27 03 00 00 00 00 00 01 00 00 00 30 2a 30 2a 30 2a b0 04 b0 04 1c | ..........'..........0*0*0*..... |
| 3780 | 00 00 d4 8c d4 01 0c 00 00 06 97 00 31 00 00 00 0c 00 01 d4 c5 00 20 03 c5 c2 00 58 02 08 07 08 | ............1..............X.... |
| 37a0 | 07 10 00 c2 33 2e 33 c2 00 58 02 60 09 60 09 16 00 c2 c3 0e c3 43 61 6c 63 75 6c 61 74 69 6f 6e | ....3.3..X.`.`.......Calculation |
| 37c0 | 20 6f 66 20 49 6e 66 65 63 74 69 76 69 74 79 20 52 65 64 75 63 74 69 6f 6e c4 0e c4 c6 28 23 60 | .of.Infectivity.Reduction....(#` |
| 37e0 | 09 c6 0a 0a c2 00 58 02 08 07 08 07 10 00 c2 c2 00 58 02 60 09 60 09 16 00 c2 57 65 6c 6c 73 20 | ......X..........X.`.`....Wells. |
| 3800 | 61 72 65 20 73 63 6f 72 65 64 20 70 6f 73 69 74 69 76 65 20 69 66 20 70 32 34 20 3e 20 33 30 20 | are.scored.positive.if.p24.>.30. |
| 3820 | 70 67 2f 6d 4c 2e 20 20 54 6f 20 63 61 6c 63 75 6c 61 74 65 0d c5 01 20 03 c5 69 6e 66 65 63 74 | pg/mL...To.calculate......infect |
| 3840 | 69 76 69 74 79 20 72 65 64 75 63 74 69 6f 6e 2c 20 70 6c 65 61 73 65 20 65 6e 74 65 72 20 70 32 | ivity.reduction,.please.enter.p2 |
| 3860 | 34 20 76 61 6c 75 65 73 20 28 72 61 77 20 64 61 74 61 29 20 69 6e 74 6f 20 74 68 65 0d 52 4c 4d | 4.values.(raw.data).into.the.RLM |
| 3880 | 50 c0 29 04 c0 20 28 44 61 74 61 77 6f 72 6b 73 20 44 65 76 65 6c 6f 70 6d 65 6e 74 2c 20 49 6e | P.)...(Dataworks.Development,.In |
| 38a0 | 63 2e 29 2c 20 75 73 69 6e 67 20 74 68 65 20 70 6c 61 73 6d 61 20 71 75 61 6e 74 69 74 61 74 69 | c.),.using.the.plasma.quantitati |
| 38c0 | 76 65 0d 6d 69 63 72 6f 63 75 6c 74 75 72 65 20 6d 6f 64 75 6c 65 20 28 70 6c 65 61 73 65 20 73 | ve.microculture.module.(please.s |
| 38e0 | 65 65 20 74 68 65 20 41 43 54 47 20 56 69 72 6f 6c 6f 67 79 20 4d 61 6e 75 61 6c 29 2e 20 20 4f | ee.the.ACTG.Virology.Manual)...O |
| 3900 | 62 74 61 69 6e 0d 49 55 50 4d 4c 20 66 6f 72 20 74 68 65 20 c0 20 04 c0 63 6f 6e 74 72 6f 6c 20 | btain.IUPML.for.the.....control. |
| 3920 | 74 65 73 74 c0 1f 04 c0 20 28 4e 48 53 29 20 61 6e 64 20 66 6f 72 20 74 68 65 20 c0 20 04 c0 74 | test.....(NHS).and.for.the.....t |
| 3940 | 65 73 74 20 73 65 74 c0 1f 04 c0 20 28 48 49 56 49 47 29 2e 20 0d d4 01 0c 00 00 06 97 00 31 00 | est.set.....(HIVIG)...........1. |
| 3960 | 78 05 0c 00 01 d4 45 78 70 72 65 73 73 20 69 6e 66 65 63 74 69 76 69 74 79 20 72 65 64 75 63 74 | x.....Express.infectivity.reduct |
| 3980 | 69 6f 6e 20 61 73 20 74 68 65 20 c3 08 c3 72 61 74 69 6f c4 08 c4 20 6f 66 20 49 55 50 4d 4c 20 | ion.as.the....ratio....of.IUPML. |
| 39a0 | 28 63 6f 6e 74 72 6f 6c 29 20 74 6f 0d 49 55 50 4d 4c 20 28 74 65 73 74 29 2e 20 20 41 20 74 65 | (control).to.IUPML.(test)...A.te |
| 39c0 | 6e 20 66 6f 6c 64 20 72 65 64 75 63 74 69 6f 6e 20 65 71 75 61 6c 73 20 39 30 25 20 6e 65 75 74 | n.fold.reduction.equals.90%.neut |
| 39e0 | 72 61 6c 69 7a 61 74 69 6f 6e 2e 20 0d 46 6f 72 20 6d 6f 72 65 20 69 6e 66 6f 72 6d 61 74 69 6f | ralization...For.more.informatio |
| 3a00 | 6e 20 61 62 6f 75 74 20 74 68 65 20 52 4c 4d 50 2c 20 70 6c 65 61 73 65 20 63 61 6c 6c 20 44 61 | n.about.the.RLMP,.please.call.Da |
| 3a20 | 74 61 77 6f 72 6b 73 20 61 74 20 32 30 36 aa 36 37 30 a9 33 38 30 36 2e c6 28 23 60 09 c6 0a 0a | taworks.at.206.670.3806..(#`.... |
| 3a40 | d4 01 0c 00 00 06 97 00 31 00 60 09 0c 00 01 d4 c2 00 58 02 08 07 08 07 10 00 c2 c2 00 58 02 60 | ........1.`.......X..........X.` |
| 3a60 | 09 60 09 16 00 c2 4e 6f 74 65 3a c2 00 58 02 b8 0b b8 0b 1c 00 c2 49 6e 20 70 72 65 70 61 72 61 | .`....Note:..X........In.prepara |
| 3a80 | 74 69 6f 6e 20 66 6f 72 20 69 6d 70 6c 65 6d 65 6e 74 69 6e 67 20 74 68 69 73 20 61 73 73 61 79 | tion.for.implementing.this.assay |
| 3aa0 | 20 69 6e 20 63 6c 69 6e 69 63 61 6c 0d 73 74 75 64 69 65 73 2c 20 6d 6f 72 65 20 64 69 73 63 75 | .in.clinical.studies,.more.discu |
| 3ac0 | 73 73 69 6f 6e 20 77 69 6c 6c 20 74 61 6b 65 20 70 6c 61 63 65 20 72 65 67 61 72 64 69 6e 67 0d | ssion.will.take.place.regarding. |
| 3ae0 | 6e 65 75 74 72 61 6c 69 7a 61 74 69 6f 6e 20 64 61 74 61 20 63 61 70 74 75 72 65 20 61 6e 64 20 | neutralization.data.capture.and. |
| 3b00 | 61 6e 61 6c 79 73 69 73 2e 20 20 45 66 66 6f 72 74 73 20 77 69 6c 6c 20 62 65 0d 6d 61 64 65 20 | analysis...Efforts.will.be.made. |
| 3b20 | 74 6f 20 69 6e 63 6f 72 70 6f 72 61 74 65 20 74 68 65 20 70 72 69 6e 63 69 70 6c 65 73 20 6f 66 | to.incorporate.the.principles.of |
| 3b40 | 20 74 68 65 20 49 44 35 30 20 70 72 6f 67 72 61 6d 0d 64 65 76 65 6c 6f 70 65 64 20 62 79 20 4a | .the.ID50.program.developed.by.J |
| 3b60 | 6f 68 6e 20 53 70 6f 75 67 65 2c 20 4d 2e 44 2e 20 20 54 68 69 73 20 70 72 6f 67 72 61 6d 20 63 | ohn.Spouge,.M.D...This.program.c |
| 3b80 | 61 6c 63 75 6c 61 74 65 73 0d 76 69 72 75 73 20 69 6e 66 65 63 74 69 6f 75 73 20 64 6f 73 65 73 | alculates.virus.infectious.doses |
| 3ba0 | 20 62 79 20 66 69 74 74 69 6e 67 20 61 20 6d 6f 64 65 6c 20 74 6f 20 74 68 65 20 64 61 74 61 2e | .by.fitting.a.model.to.the.data. |
| 3bc0 | 20 20 49 74 0d 6f 62 74 61 69 6e 73 20 6c 6f 67 20 49 44 35 30 20 66 6f 72 20 74 68 65 20 c0 20 | ..It.obtains.log.ID50.for.the... |
| 3be0 | 04 c0 63 6f 6e 74 72 6f 6c 20 73 65 74 c0 1f 04 c0 20 28 4e 48 53 29 20 61 6e 64 20 66 6f 72 20 | ..control.set.....(NHS).and.for. |
| 3c00 | 74 68 65 0d c0 20 04 c0 74 65 73 74 20 73 65 74 c0 1f 04 c0 20 28 48 49 56 49 47 29 2e 20 20 49 | the.....test.set.....(HIVIG)...I |
| 3c20 | 6e 66 65 63 74 69 76 69 74 79 20 72 65 64 75 63 74 69 6f 6e 20 69 73 20 65 78 70 72 65 73 73 65 | nfectivity.reduction.is.expresse |
| 3c40 | 64 20 61 73 0d d4 01 0c 00 00 06 97 00 31 00 a0 0f 0c 00 01 d4 74 68 65 20 c3 08 c3 64 69 66 66 | d.as.........1.......the....diff |
| 3c60 | 65 72 65 6e 63 65 c4 08 c4 20 62 65 74 77 65 65 6e 20 6c 6f 67 49 44 35 30 20 28 63 6f 6e 74 72 | erence....between.logID50.(contr |
| 3c80 | 6f 6c 29 20 61 6e 64 20 6c 6f 67 49 44 35 30 20 28 74 65 73 74 29 2e 20 0d 4f 6e 65 20 6c 6f 67 | ol).and.logID50.(test)...One.log |
| 3ca0 | 20 72 65 64 75 63 74 69 6f 6e 20 65 71 75 61 6c 73 20 39 30 25 20 6e 65 75 74 72 61 6c 69 7a 61 | .reduction.equals.90%.neutraliza |
| 3cc0 | 74 69 6f 6e 2e 20 20 46 6f 72 20 6d 6f 72 65 0d 69 6e 66 6f 72 6d 61 74 69 6f 6e 20 61 62 6f 75 | tion...For.more.information.abou |
| 3ce0 | 74 20 74 68 69 73 20 70 72 6f 67 72 61 6d 2c 20 70 6c 65 61 73 65 20 63 61 6c 6c 20 44 72 2e 20 | t.this.program,.please.call.Dr.. |
| 3d00 | 53 70 6f 75 67 65 20 61 74 0d 33 30 31 a9 34 39 36 a9 32 34 37 35 2e c6 28 23 b8 0b c6 0a | Spouge.at.301.496.2475..(#.... |
 |
index : libextractor.git | |
| GNU libextractor |
| aboutsummaryrefslogtreecommitdiff |
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